Novel tyrosine kinase identified by phosphotyrosine antibody screening of cDNA libraries.
Novel tyrosine kinase identified by phosphotyrosine antibody screening of cDNA libraries.
复制标题
通过 cDNA 文库的磷酸酪氨酸抗体筛选鉴定出新型酪氨酸激酶。
DOI：
10.1128/mcb.8.12.5541-5544.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Hanafusa,H
中科院分区：
文献类型：
--
作者：
Kornbluth,S;Paulson,KE;Hanafusa,H
为了克隆蛋白酪氨酸激酶，用抗磷酸化酪氨酸抗体筛选λgt11c DNA表达文库。利用该方法，从小鼠肝脏cDNA文库中克隆了编码一种新的酪氨酸激酶的全长2.5kb的cDNA。这个新基因与受体酪氨酸激酶、FMS和KIT关系最密切。
In an attempt to clone protein tyrosine kinases, antiphosphotyrosine antibodies were used to screen λgt11 cDNA expression libraries. By this method, a 2.5-kilobase cDNA encoding a novel tyrosine kinase was isolated from a mouse liver cDNA library. This new gene is most closely related to the receptor tyrosine kinasesret,fms, andkit.