ARE BETA-1 INTEGRINS INVOLVED IN XENOPUS GASTRULATION
ARE BETA-1 INTEGRINS INVOLVED IN XENOPUS GASTRULATION
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DOI：
10.1016/0925-4773(92)90003-3
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发表时间：
1992-08-01
影响因子：
2.6
通讯作者：
SMITH, JC
中科院分区：
文献类型：
--
作者：
HOWARD, JE;HIRST, EMA;SMITH, JC
脊椎动物原肠胚形成的分子基础知之甚少。对有尾目两栖动物的研究涉及含有β-1的整合素，但对非洲爪蟾可用的有限信息表明并非如此：含有RGD序列的肽不抑制原肠胚形成，并且通过激活素诱导假定外胚层细胞中的细胞扩散不伴随整合素β-1合成的增加。在这里，我们报告，β-1-含有整合素，然而，主要的纤连蛋白受体在非洲爪蟾原肠胚，虽然他们的细胞表面水平低。与含有RGD位点的肽不同，识别该分子外部结构域的抗体在引入囊胚腔时可以阻断原肠胚形成。这些结果使我们能够提出一个模型来解释整合素β 1在非洲爪蟾原肠胚形成中的作用。
The molecular basis of vertebrate gastrulation is poorly understood. Work on urodele amphibians has implicated beta-1-containing integrins, but th limited information available for Xenopus indicates otherwise: peptides containing the RGD sequence do not inhibit gastrulation and induction of cell spreading in presumptive ectodermal cells by activin is not accompanied by an increase in synthesis of integrin beta-1. Here we report that beta-1-containing integrins are, nevertheless, the principal fibronectin receptors in the Xenopus gastrula, although their cell surface levels are low. Antibodies recognizing the external domain of the molecule can, unlike peptides containing the RGD site, block gastrulation when introduced into the blastocoel. These results allow us to propose a model to explain the role of integrin beta-1, in Xenopus gastrulation.