Bovine chromaffin cells have insulin-like growth factor-I (IGF-I) receptors: IGF-I enhances catecholamine secretion.
Bovine chromaffin cells have insulin-like growth factor-I (IGF-I) receptors: IGF-I enhances catecholamine secretion.
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牛嗜铬细胞具有胰岛素样生长因子-I (IGF-I) 受体：IGF-I 增强儿茶酚胺分泌。
DOI：
10.1111/j.1471-4159.1988.tb04873.x
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发表时间：
1988
影响因子：
4.7
通讯作者：
Perlman,RL
中科院分区：
文献类型：
--
作者：
Dahmer,MK;Perlman,RL
测定了125I-胰岛素样生长因子-I(125I-IGF-I)与牛嗜铬细胞的结合。嗜铬细胞培养含111,000±40,000个IGF-I结合位点/细胞。这些结合位点与IGF-I结合的AKD为1.1±0.3nM，与胰岛素的亲和力较低。交叉连接研究表明，125I-IGFI结合到一种具有∼125,000分子量的蛋白质上，类似于其他组织中IGFI型受体的α亚单位的分子量。细胞经IGF-I(10 NM)培养4天后，高K+诱发的儿茶酚胺分泌量几乎增加一倍。胰岛素在促进儿茶酚胺分泌方面比IGF-I弱得多。这些数据表明，IGF-I与嗜铬细胞上的IGF-I受体结合可以调节这些细胞的功能。
The binding of125I‐insulin‐like growth factor‐I (125I‐IGF‐I) to bovine chromaffin cells was measured. Chromaffin cell cultures contained 111,000 ± 40,000 IGF‐I binding sites/cell. These sites bound IGF‐I with aKDof 1.1 ± 0.3 nMand had a much lower affinity for insulin. Cross‐linking studies showed that125I‐IGF‐I bound to a protein that had an Mrof ∼ 125,000, similar to the Mrof the α subunit of the IGF‐I receptor in other tissues. Cells cultured with IGF‐I (10 nM) for 4 days exhibited an almost twofold increase in high K+‐evoked catecholamine secretion. Insulin was much less potent than IGF‐I in enhancing catecholamine secretion. These data indicate that binding of IGF‐I to its receptors on chromaffin cells can modulate the function of these cells.