Comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from water
Comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from water
复制标题
木质素过氧化物酶与辣根过氧化物酶催化去除水中壬基酚的比较
DOI：
10.1007/s11356-013-2161-4
复制
发表时间：
2014-02-01
影响因子：
5.8
通讯作者：
Gao, Shixiang
中科院分区：
文献类型：
--
作者：
Dong, Shipeng;Mao, Liang;Gao, Shixiang
水相壬基酚（NP），一个众所周知的内分泌干扰化学品的浓度，被证明是有效地减少通过反应与木质素过氧化物酶（LiP）或辣根过氧化物酶（HRP）和过氧化氢。我们系统地评估了它们在不同条件下的反应效率，结果证实，在实验条件下，LiP对NP的催化性能比HRP更有效。质谱分析表明，通过自由基-自由基偶联机理的聚合是导致NP转化的途径。我们的从头算的协助下的分子模拟表明，NP的偶联可能通过两个NP自由基之间的共价键在其未取代的碳酚环。急性制动试验数据与水蚤确认，NP毒性有效地消除了LiP/HRP催化的NP去除。本研究的结果为理解LiP/HRP介导的NP反应提供了有用的信息，并且酶性能的比较可以展示其在水/废水处理中的大规模应用的优势。
Concentrations of aqueous-phase nonylphenol (NP), a well-known endocrine-disrupting chemical, are shown to be reduced effectively via reaction with lignin peroxidase (LiP) or horseradish peroxidase (HRP) and hydrogen peroxide. We systematically assessed their reaction efficiencies at varying conditions, and the results have confirmed that the catalytic performance of LiP toward NP was more efficient than that of HRP under experimental conditions. Mass spectrum analysis demonstrated that polymerization through radical–radical coupling mechanism was the pathway leading to NP transformation. Our molecular modeling with the assistance of ab initio suggested the coupling of NP likely proceeded via covalent bonding between two NP radicals at their unsubstituted carbons in phenolic rings. Data from acute immobilization tests withDaphniaconfirm that NP toxicity is effectively eliminated by LiP/HRP-catalyzed NP removal. The findings in this study provide useful information for understanding LiP/HRP-mediated NP reactions, and comparison of enzymatic performance can present their advantages for up-scale applications in water/wastewater treatment.