Isolation of tropoelastin a from lathyritic chick aortae.
Isolation of tropoelastin a from lathyritic chick aortae.
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从山豆状小鸡主动脉中分离原弹性蛋白 a。
DOI：
10.1042/bj2170581
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Foster,JA
中科院分区：
文献类型：
--
作者：
Rich,CB;Foster,JA
采用严格的变性条件从鸡胚中提取原弹性蛋白a。这种新物种的原弹性蛋白的氨基酸组成是弹性蛋白样的，其高比例的脯氨酸，甘氨酸，丙氨酸和缬氨酸。然而，它与真正的弹性蛋白原B的不同之处在于含有更高百分比的极性氨基酸和半胱氨酸残基。此外，脯氨酸羟基化的量比在鸡原弹性蛋白B中发现的高3倍。
Tropoelastin a was isolated from lathyritic chick aortae by using severe denaturing conditions for the initial extraction. The amino acid composition of this new species of tropoelastin is elastin-like in its high proportion of proline, glycine, alanine and valine. However, it differs from authentic tropoelastin b in containing a higher percentage of polar amino acids and cysteine residues. In addition, the amount of proline hydroxylation is 3 times higher than that found in chick tropoelastin b.