Long non-coding RNA MEG3 silencing protects against light-induced retinal degeneration.
Long non-coding RNA MEG3 silencing protects against light-induced retinal degeneration.
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DOI：
10.1016/j.bbrc.2018.01.177
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发表时间：
2018-02
影响因子：
3.1
通讯作者：
Yunxi Zhu;Jin Yao;Chang Liu;Haitao Hu;Xiu-Miao Li;Hui-min Ge;Yunfan Zhou;Kun Shan;Qin Jiang-Qin-Jia
中科院分区：
文献类型：
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作者：
Yunxi Zhu;Jin Yao;Chang Liu;Haitao Hu;Xiu-Miao Li;Hui-min Ge;Yunfan Zhou;Kun Shan;Qin Jiang-Qin-Jia
过度光照会导致视网膜变性，并加速多种眼部疾病的进展和严重程度，例如年龄相关性黄斑变性 (AMD) 和色素性视网膜炎。长非编码 RNA (LncRNA) 已成为光感受器发育和眼部疾病的重要调节因子。在这项研究中，我们研究了 lncRNA-MEG3 在光诱导的视网膜变性中的作用。体内和体外光损伤后 MEG3 表达显着上调。 MEG3 沉默可防止体内光诱导的视网膜变性和体外光诱导的感光细胞凋亡。从机制上讲，MEG3 通过充当 p53 诱饵来调节视网膜感光细胞功能。 MEG3 沉默降低了 caspase 3/7 活性，上调抗凋亡蛋白 (Bcl-2) 表达，并下调促凋亡蛋白 (Bax) 表达。总而言之，这项研究提供了一种有前途的 MEG3 沉默方法，用于治疗光诱导的视网膜变性。
Excessive light exposure leads to retinal degeneration and accelerates the progression and severity of several ocular diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa. Long non-coding RNAs (LncRNAs) have emerged as important regulators of photoreceptor development and ocular diseases. In this study, we investigated the role of lncRNA-MEG3 in light-induced retinal degeneration. MEG3 expression was significantly up-regulated after light insult in vivo and in vitro. MEG3 silencing protected against light-induced retinal degeneration in vivo and light-induced photoreceptor cell apoptosis in vitro. Mechanistically, MEG3 regulated retinal photoreceptor cell function by acting as p53 decoy. MEG3 silencing decreased caspase 3/7 activity, up-regulated anti-apoptotic protein (Bcl-2) expression, and down-regulated pro-apoptotic protein (Bax) expression. Taken together, this study provides a promising method of MEG3 silencing for treating light-induced retinal degeneration.