Carnosic Acid Inhibits CXCR3 Ligands Production in IL-27-Stimulated Human Oral Epithelial Cells
Carnosic Acid Inhibits CXCR3 Ligands Production in IL-27-Stimulated Human Oral Epithelial Cells
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DOI：
10.1007/s10753-019-00991-6
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发表时间：
2019-08-01
期刊：
影响因子：
5.1
通讯作者：
Matsuo, Takashi
中科院分区：
文献类型：
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作者：
Hosokawa, Ikuko;Hosokawa, Yoshitaka;Matsuo, Takashi
鼠尾草酸是从迷迭香中分离得到的一种生物活性化合物，具有多种药理作用。然而，鼠尾草酸对牙周炎的抗炎作用仍不清楚。本研究旨在探讨鼠尾草酸对IL-27刺激的人口腔上皮细胞（TR 146细胞）中参与Th 1细胞迁移、聚集和分泌的CXC趋化因子受体3（CXCR 3）配体的影响。鼠尾草酸以剂量依赖性方式降低IL-27刺激的TR 146细胞中CXC趋化因子配体（CXCL）9、CXCL 10和CXCL 11的产生。此外，我们发现鼠尾草酸可以抑制IL-27刺激的TR 146细胞中的信号转导和转录激活因子（STAT）1、STAT 3和蛋白激酶B（Akt）磷酸化。此外，STAT 1、STAT 3和Akt抑制剂可抑制IL-27处理的TR 146细胞中CXCR 3配体的产生。因此，鼠尾草酸可通过抑制STAT 1、STAT 3和Akt的活化，减少人口腔上皮细胞CXCR 3配体的产生。
Carnosic acid, which is a bioactive compound isolated from rosemary, has various pharmacological effects. However, the anti-inflammatory effect of carnosic acid on periodontitis is still unknown. The aim of this study was to investigate the effect of carnosic acid on CXC chemokine receptor 3 (CXCR3) ligands, which are involved in Th1 cells migration and accumulation, production in interleukin (IL)-27-stimulated human oral epithelial cells (TR146 cells). Carnosic acid decreased CXC chemokine ligand (CXCL)9, CXCL10, and CXCL11 production in IL-27-stimulated TR146 cells in a dose-dependent fashion. Moreover, we disclosed that carnosic acid could suppress signal transducer and activator of transcription (STAT)1, STAT3, and protein kinase B (Akt) phosphorylation in IL-27-stimulated TR146 cells. Furthermore, STAT1, STAT3, and Akt inhibitors could suppress CXCR3 ligands production in IL-27-treated TR146 cells. In summary, carnosic acid could reduce CXCR3 ligands production in human oral epithelial cell by inhibiting STAT1, STAT3, and Akt activation.