Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area.
Rickettsial infection in Amblyomma cajennense ticks and capybaras (Hydrochoerus hydrochaeris) in a Brazilian spotted fever-endemic area.
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DOI：
10.1186/1756-3305-7-7
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发表时间：
2014-01-05
影响因子：
3.2
通讯作者：
Labruna MB
中科院分区：
文献类型：
--
作者：
Krawczak FS;Nieri-Bastos FA;Nunes FP;Soares JF;Moraes-Filho J;Labruna MB
巴西斑疹热 (BSF) 由立克次体细菌引起，是世界上最致命的斑疹热。在大多数BSF流行地区，水豚（Hydrochoerus Hydrochaeris）是Amblyomma cajennense蜱的主要宿主，而Amblyomma cajennense蜱是BSF的主要媒介。 2012年，巴西东南部伊图市的一个住宅公园内有受A. cajennense感染的水豚居住的住宅公园内，一名儿童被蜱虫叮咬，确诊了一起BSF病例。寻找寄主的 A. cajennense 成年蜱在住宅公园被收集，并活体带到实验室，在那里将它们浸渍并腹膜内接种到豚鼠中。一只表现出高烧的接种蜱的豚鼠被安乐死，其内脏被浸渍并接种到另外的豚鼠中（豚鼠传代）。豚鼠传代的组织样本也被用于通过壳瓶技术接种 Vero 细胞。通过对三个立克次体基因（gltA、ompA 和 ompB）片段进行 PCR 和 DNA 测序，使用受感染的细胞对立克次体分离株进行分子表征。从居住在该住宅公园的 172 只水豚身上采集了血清样本。使用立克次体抗原通过免疫荧光测定法测试血清。一只接种蜱虫的豚鼠出现高烧并伴有阴囊反应（水肿和明显发红）。这些迹象通过连续的豚鼠传代而重现。在接种来自第三代发热豚鼠的脑匀浆的 Vero 细胞中成功分离出立克次体。该立克次体分离株（命名为 ITU 菌株）的分子表征产生的 DNA 序列与 Genbank 中立克次体相应序列 100% 相同。 172 只水豚中共有 83 只 (48.3%) 对立氏立克次氏体有血清反应，终点滴度范围为 64 至 8192。从 A. cajennense 蜱中获得了立克次氏立克次体的活分离株，这是过去 60 年来从该蜱种中分离出的第一个活的立克次氏立克次体分离株。住宅公园近一半的水豚种群对立氏立克次体有血清反应，证实了当地水豚种群被立克次体感染的发现。
Brazilian spotted fever (BSF), caused by the bacterium Rickettsia rickettsii, is the deadliest spotted fever of the world. In most of the BSF-endemic areas, capybaras (Hydrochoerus hydrochaeris) are the principal host for the tick Amblyomma cajennense, which is the main vector of BSF. In 2012, a BSF case was confirmed in a child that was bitten by ticks in a residential park area inhabited by A. cajennense-infested capybaras in Itú municipality, southeastern Brazil. Host questing A. cajennense adult ticks were collected in the residential park and brought alive to the laboratory, where they were macerated and intraperitoneally inoculated into guinea pigs. A tick-inoculated guinea pig that presented high fever was euthanized and its internal organs were macerated and inoculated into additional guinea pigs (guinea pig passage). Tissue samples from guinea pig passages were also used to inoculate Vero cells through the shell vial technique. Infected cells were used for molecular characterization of the rickettsial isolate through PCR and DNA sequencing of fragments of three rickettsial genes (gltA, ompA, and ompB). Blood serum samples were collected from 172 capybaras that inhabited the residential park. Sera were tested through the immunofluorescence assay using R. rickettsii antigen. A tick-inoculated guinea pig presented high fever accompanied by scrotal reactions (edema and marked redness). These signs were reproduced by consecutive guinea pig passages. Rickettsia was successfully isolated in Vero cells that were inoculated with brain homogenate derived from a 3rd passage-febrile guinea pig. Molecular characterization of this rickettsial isolate (designated as strain ITU) yielded DNA sequences that were all 100% identical to corresponding sequences of R. rickettsii in Genbank. A total of 83 (48.3%) out of 172 capybaras were seroreactive to R. rickettsii, with endpoint titers ranging from 64 to 8192. A viable isolate of R. rickettsii was obtained from the tick A. cajennense, comprising the first viable R. rickettsi isolate from this tick species during the last 60 years. Nearly half of the capybara population of the residential park was seroreactive to R. rickettsii, corroborating the findings that the local A. cajennense population was infected by R. rickettsii.
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影响因子：
3.1
作者：
HAYES, SF;BURGDORFER, W
通讯作者：
BURGDORFER, W
影响因子：
11.8
作者：
Hidalgo M;Orejuela L;Fuya P;Carrillo P;Hernandez J;Parra E;Keng C;Small M;Olano JP;Bouyer D;Castaneda E;Walker D;Valbuena G
通讯作者：
Valbuena G
DOI：
10.1111/j.1749-6632.2009.04516.x
发表时间：
2009-01-01
期刊：
RICKETTSIOLOGY AND RICKETTSIAL DISEASES
影响因子：
--
作者：
Labruna, Marcelo B.
通讯作者：
Labruna, Marcelo B.
DOI：
10.4269/ajtmh.1970.19.1010
发表时间：
1970-01-01
影响因子：
3.3
作者：
BURGDORFER, W
通讯作者：
BURGDORFER, W
DOI：
10.4269/ajtmh.2012.12-0153
发表时间：
2012-08-01
影响因子：
3.3
作者：
Argueello, Ana Patricia;Hun, Laya;Taylor, Lizeth
通讯作者：
Taylor, Lizeth