Immobilization of glucose oxidase in electrospun nanofibrous membranes for food preservation
Immobilization of glucose oxidase in electrospun nanofibrous membranes for food preservation
复制标题
电纺纳米纤维膜中葡萄糖氧化酶的固定化用于食品保鲜
DOI：
10.1016/j.foodcont.2012.01.022
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发表时间：
2012-07-01
期刊：
影响因子：
6
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Ge, Ling;Zhao, Yu-sheng;Li, Ping
将葡萄糖氧化酶固定在PVA/CS/茶提取物静电纺纳米纤维膜上，制备了一种新型的食品包装材料。本文采用菌落计数法对复合膜的抑菌活性进行了测试。利用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）对静电纺纳米纤维的形貌和结构进行了表征。傅里叶变换红外光谱证实了固定化葡萄糖氧化酶在电纺丝纳米纤维膜上的存在，固定化酶的最佳活性是游离酶的68%以上。葡萄糖氧化酶固定化的静电纺丝膜对样品的脱氧率为73%，因为当氧浓度为1时，微生物的生长和繁殖受到明显抑制
A novel food packaging material was obtained by immobilizing glucose oxidase in PVA/CS/tea extract electrospun nanofibrous membrane. In this paper, the antibacterial activity of the composite membrane was tested by colony counting method. The morphology and structure of the electrospun nanofibers were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR), respectively. FT-IR confirmed the existence of glucose oxidase immobilized in the electrospun nanofibrous membrane, the optimal activity of the immobilize enzyme was over 68% to that of the free enzyme. The electrospun membrane immobilized with glucose oxidase exerted 73% of deoxidization to the tested samples, as the growth and reproduction of microorganisms were significantly inhibited when the oxygen concentration was