The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates
The power of two: An artificial microbial consortium for the conversion of inulin into Polyhydroxyalkanoates
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DOI：
10.1016/j.ijbiomac.2021.08.123
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发表时间：
2021-08-26
影响因子：
8.2
通讯作者：
Pezzella, Cinzia
中科院分区：
文献类型：
--
作者：
Corrado, Iolanda;Petrillo, Claudia;Pezzella, Cinzia
微生物生产聚羟基链烷酸酯（PHA）的主要问题之一是确保可再生的非食品生物质原料供给发酵过程。菊粉是一种多分散的果聚糖，在几种低需求作物的根部积累作为储备多糖，有可能面对这一挑战。筛选芽孢杆菌属物种的微生物收集物以获得有效的菊粉酶生产者，并分析所选菌株RHF 15（鉴定为吉氏芽孢杆菌）的基因组，以揭示其广泛的分解代谢潜力。RHF 15与钩虫贪铜菌（Cupriavidus necator）共培养，钩虫贪铜菌是一种成熟的PHA生产菌，缺乏代谢菊粉的能力。应用中心复合旋转设计（CCRD）优化PHA合成菊糖的人工微生物财团，评估物种接种率和菊糖和Nsource浓度的影响。在优化条件下，聚羟基丁酸酯（Polyhydroxybutyrate，简称PHB）的最大产率为1.9 g L-1，相当于聚合物含量的80%（g（polymer）/g（CDW））。研究的方法代表了一种有效的工艺优化方法，可能适用于从其他复杂的碳源生产PHA。
One of the major issues for the microbial production of polyhydroxyalkanoates (PHA) is to secure renewable, non-food biomass feedstocks to feed the fermentation process. Inulin, a polydisperse fructan that accumulates as reserve polysaccharide in the roots of several low-requirement crops, has the potential to face this challenge.In this work, a "substrate facilitator" microbial consortium was designed to address PHA production using inulin as feedstock. A microbial collection of Bacillus species was screened for efficient inulinase producer and the genome of the selected strain, RHF15, identified as Bacillus gibsonii, was analysed unravelling its wide catabolic potential. RHF15 was co-cultured with Cupriavidus necator, an established PHA producer, lacking the ability to metabolize inulin. A Central Composite Rotary Design (CCRD) was applied to optimise PHA synthesis from inulin by the designed artificial microbial consortium, assessing the impact of species inoculum ratio and inulin and Nsource concentrations. In the optimized conditions, a maximum of 1.9 g L-1 of Polyhydroxybutyrate (PHB), corresponding to similar to 80% (g(polymer)/g(CDW)) polymer content was achieved. The investigated approach represents an effective process optimization method, potentially applicable to the production of PHA from other complex Csources.