DELETION AND TRANSPOSON MUTAGENESIS AND SEQUENCE-ANALYSIS OF THE PR01600 ORIR REGION FOUND IN THE BROAD-HOST-RANGE PLASMIDS OF THE PQF SERIES
DELETION AND TRANSPOSON MUTAGENESIS AND SEQUENCE-ANALYSIS OF THE PR01600 ORIR REGION FOUND IN THE BROAD-HOST-RANGE PLASMIDS OF THE PQF SERIES
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DOI：
10.1006/plas.1994.1028
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发表时间：
1994-05-01
期刊：
影响因子：
2.6
通讯作者：
KROPINSKI, AM
中科院分区：
文献类型：
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作者：
JANSONS, I;TOUCHIE, G;KROPINSKI, AM
已确定了小隐蔽广宿主范围质粒pRO 1600的复制起点（OriR）区的核苷酸序列，该质粒形成了许多有用的克隆载体的基础。此外，它已进行Tn 5诱变，缺失分析，亚克隆，以确定在铜绿假单胞菌复制所必需的区域。序列（1894 bp）含有衍生自转座子Tnl的片段。OriR区域在结构上与其他复制（Rep）蛋白依赖性起点相关，因为它在4个17 bp的同向重复序列（iteron）上游具有富含A-T的区域，推测其在起始蛋白结合中起作用。该序列还含有DNA-A结合位点和开放阅读框，其可以编码与来自淋病奈瑟菌β-内酰胺酶质粒pFA 3的RepA同源的碱性（pI 10.6）25，343-Da Rep蛋白。讨论了铜绿假单胞菌（RP 1）中该质粒可能的进化起源。(C)1994年出版社出版。
The nucleotide sequence of the replicative origin (OriR) region of the small cryptic broad-host-range plasmid, pRO1600, which forms the basis of a number of useful cloning Vectors has been determined. In addition it has been subjected to Tn5 mutagenesis, deletion analysis, and subcloning in order to define the regions essential for replication in Pseudomonas aeruginosa. The sequence (1894 bp) contains a fragment derived from transposon Tnl. The OriR region is structurally related to other replication (Rep) protein-dependent origins in that it has an A-T-rich region upstream of four 17-bp direct repeats (iterons) which presumably function in initiator protein binding. The sequence also contains a DNA-A-binding site and an open reading frame which could encode a basic (pI 10.6) 25,343-Da Rep protein with homology to RepA from the Neisseria gonorrhoeae beta-lactamase plasmid pFA3. The possible evolutionary origin of this plasmid in P. aeruginosa (RPl) is discussed. (C) 1994 Academic Press, Inc.