Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.
Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.
复制标题
人嗜酸性粒细胞颗粒富含精氨酸的阳离子蛋白：嗜酸性粒细胞和中性粒细胞的成分比较。
DOI：
--
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
Lehrer Ri
中科院分区：
文献类型：
--
作者：
I. Olsson;P. Venge;Spitznagel Jk;Lehrer Ri
先前从白血病骨髓细胞颗粒中分离出的几种富含精氨酸的阳离子蛋白已被发现主要存在于人嗜酸性粒细胞中。主要成分的分子量为21，000，每摩尔蛋白质含有约2.6摩尔锌。对显著嗜酸性粒细胞增多患者白细胞的细胞质进行高速离心，结果表明，这组蛋白质被包装在含有晶体的大嗜酸性粒细胞颗粒中。嗜酸性粒细胞颗粒中约30%的蛋白质含量属于这组阳离子蛋白。未检测到阳离子蛋白的杀菌或酯溶解活性，也未抑制豚鼠过敏毒素或组胺诱导的收缩。先前在豚鼠嗜酸性粒细胞中证实的碱性蛋白可能类似于人嗜酸性粒细胞的碱性蛋白组，但发现分子量和氨基酸组成存在很大差异。
Several arginine-rich cationic proteins previously isolated from granules of leukemic myeloid cells have been found to reside primarily in human eosinophil leukocytes. The major component has a molecular weight of 21,000 and it contains approximately 2.6 moles of zinc per mole of protein. Velocity centrifugation of cytoplasm from leukocytes of patients with marked eosinophilia showed that this group of proteins is packaged in the crystalloid-containing large eosinophil granules. Approximately 30% of the protein content of eosinophil granules belonged to this group of cationic proteins. Bactericidal or esterolytic activities of the cationic proteins were not detected, nor did they inhibit guinea pig anaphylatoxin or histamine-induced contraction. The basic protein previously demonstrated in guinea pig eosinophils may be analogous to the group of basic proteins of human eosinophils but great differences are found for molecular weight and amino acid composition.