Nanomechanical motion of Escherichia coli adhered to a surface
Nanomechanical motion of Escherichia coli adhered to a surface
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DOI：
10.1063/1.4895132
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发表时间：
2014-09-15
影响因子：
4
通讯作者：
Ekinci, K. L.
中科院分区：
文献类型：
--
作者：
Lissandrello, C.;Inci, F.;Ekinci, K. L.
利用微悬臂梁研究了附着在化学功能化硅表面的细菌的纳米机械运动。使用非特异性结合剂将大肠杆菌(E.coli)附着到硅微悬臂的表面。微悬臂梁保持在液体介质中，并使用光学位移传感器监测其纳米机械波动。细菌的运动有效地耦合到远低于其共振频率的微悬臂梁上，导致微悬臂梁波动的可测量的增加。在时间域中，波动表现出大幅度的低频振荡。在相应的频域测量中，观察到机械能集中在低频，遵循1/f(α)型幂规律。用一个基本的物理模型来解释观测到的机械能的光谱分布。这些结果为理解附着在表面的微生物的运动和开发细菌微机械传感器奠定了基础。(C)2014 AIP出版有限责任公司。
Nanomechanical motion of bacteria adhered to a chemically functionalized silicon surface is studied by means of a microcantilever. A non-specific binding agent is used to attach Escherichia coli (E. coli) to the surface of a silicon microcantilever. The microcantilever is kept in a liquid medium, and its nanomechanical fluctuations are monitored using an optical displacement transducer. The motion of the bacteria couples efficiently to the microcantilever well below its resonance frequency, causing a measurable increase in the microcantilever fluctuations. In the time domain, the fluctuations exhibit large-amplitude low-frequency oscillations. In corresponding frequency-domain measurements, it is observed that the mechanical energy is focused at low frequencies with a 1/f(alpha)-type power law. A basic physical model is used for explaining the observed spectral distribution of the mechanical energy. These results lay the groundwork for understanding the motion of microorganisms adhered to surfaces and for developing micromechanical sensors for bacteria. (C) 2014 AIP Publishing LLC.