Analysis of differential gene expression in plurihormonal pituitary adenomas using bead-based fiber-optic arrays
Analysis of differential gene expression in plurihormonal pituitary adenomas using bead-based fiber-optic arrays
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DOI：
10.1007/s11060-011-0792-1
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发表时间：
2012-07-01
影响因子：
3.9
通讯作者：
Zhang, Yazhuo
中科院分区：
文献类型：
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作者：
Jiang, Zhiquan;Gui, Songbai;Zhang, Yazhuo
多激素垂体腺瘤（PHPA）是指分泌两种或两种以上化学成分、免疫反应性和生物学效应不同的激素的垂体腺瘤。由于这些腺瘤的发病机制还不清楚，我们的研究旨在探讨PHPA发病机制的基础。我们使用基于珠的光纤阵列（Illumina人类基因芯片WG-6 v3.0），以检查基因表达谱在7 PHPA相比，三个正常的垂体腺。随机选择4个差异表达基因，通过实时定量逆转录聚合酶链反应进行验证。然后，我们使用京都基因和基因组百科全书对所有差异表达的基因进行通路分析。我们的阵列分析显示，在PHPA中，6个基因的表达显著增加，334个基因和15个表达序列标签的表达显著减少。生物信息学分析表明，基因HIGD 1B、EPS 8、ECT 2和BTG 2可能在PHPA的发生发展中起重要作用。通路分析表明，p53和Notch信号通路可能在PHPA的发生发展中发挥重要作用，细胞外基质（ECM）-受体相互作用可能在抑制PHPA侵袭和转移中发挥作用。我们的数据表明，有许多异常表达的基因和途径参与PHPA的发病机制。基于微珠的光纤阵列结合基因表达数据的通路分析似乎是研究肿瘤发病机制的有效方法。
Plurihormonal pituitary adenomas (PHPAs) are defined as those pituitary adenomas secreting two or more hormones that differ in chemical composition, immunoreactivity, and biologic effects. Since the pathogenesis of these adenomas is not well understood, our study aimed to explore mechanisms underlying the pathogenesis of PHPAs. We used bead-based fiber-optic arrays (Illumina Human GeneChip WG-6 v3.0) to examine the gene expression profiles in seven PHPAs compared with three normal pituitary glands. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse-transcription polymerase chain reaction. We then performed pathway analysis of all differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Our array analysis showed significant increases in the expression of 6 genes and decreases in 334 genes and 15 expressed sequence tags in the PHPAs. Bioinformatic analysis showed that genes HIGD1B, EPS8, ECT2, and BTG2 might play an important role in the tumorigenesis and progression of PHPAs. Pathway analysis showed that the p53 and Notch signaling pathways may play an important role in tumorigenesis and progression of PHPAs, and extracellular matrix (ECM)-receptor interactions likely play a role in the inhibition of invasion and metastasis in these tumors. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of PHPAs. Bead-based fiber-optic arrays combined with pathway analysis of gene expression data appears to be a valid method for investigating the pathogenesis of tumors.