A non-lymphoma idiotype is indicative and predictive for B cell malignancies in AIDS.
A non-lymphoma idiotype is indicative and predictive for B cell malignancies in AIDS.
复制标题
非淋巴瘤独特型可指示和预测 AIDS 中的 B 细胞恶性肿瘤。
作者：
Herndier,B;McGrath,M;Abbey,N;Wang,HT;Ng,V;Dorigo,O;Kaplan,L;Muller,S;Kohler,H
先前定义的1F7独特型(JImmunol 147：933；1991和Eur JImmunol 22：1749；1992)在与HIV的不同蛋白质(gp41、p24、gp120)反应的抗体上表达。由于 HIV 淋巴瘤患者的 1F7（IgM 或 IgG）血清水平显着高于 HIV 感染者、正常对照和非 HIV 淋巴瘤患者，因此我们假设 B 细胞肿瘤是独特型的来源。然而，低温切片的免疫组织化学显示肿瘤性 B 细胞和肿瘤浸润性 T 细胞上均没有 1F7 独特型信号 (n=8)。有趣的是，肿瘤块附近的反应性淋巴细胞增多和反应性滤泡增生对照（n=5）表现出显着的 1F7 反应性。反应性出现在副皮质和滤泡周围淋巴区域，主要是淋巴结或扁桃体中的 B、T 和抗原呈递细胞区域。对源自 HIV 感染者的具有抗 HIV 特异性的电泳确定的副蛋白进行的调查显示，与 1F7 抗独特型抗体没有蛋白质印迹反应性。因此，独特型似乎不是 B 细胞肿瘤或异常 B 细胞增殖的直接产物，而是由响应 HIV 感染的 B 细胞克隆产生的。这种高水平的血清 1F7 反应性可能是 HIV 淋巴瘤发病机制的重要线索，并为 HIV 淋巴瘤提供高度预测性的血清学检测。
The 1F7 idiotype previously defined (J Immunol 147:933; 1991 and Eur J Immunol 22:1749; 1992) is expressed on antibodies reactive to different proteins of HIV (gp41, p24, gpl20). Since serum levels of 1F7 (IgM or IgG) are significantly higher in patients with HIV lymphoma as opposed to HIV-infected individuals, normal controls and non-HIV lymphoma patients, we hypothesized the B-cell neoplasms were the source of the idiotype. However, immunohistochemistry on cryostat sections revealed no 1F7 idiotype signal on neoplastic B-cells nor tumor infiltrative T-cells (n=8). Interestingly, reactive lymphocytosis adjacent to tumor masses and reactive follicular hyperplastic controls (n=5) exhibited significant 1F7 reactivity. The reactivity appeared in paracortical and perifollicular lymphoid regions, predominantly regions of B, T and antigen presenting cells in lymph nodes or tonsils. A survey of electrophoretically defined paraproteins with anti-HlV specificities derived from HIV-infected patients showed no Western blot reactivity with the 1F7 anti-idiotypic antibody. Therefore, the idiotype does not appear to be a direct product of B-cell neoplasia or abnormal B-cell proliferation, but is produced by B cell clones responding to HIV infection. This high level of serum 1F7 reactivity could be an important clue in the pathogenesis of HIV lymphomas and confers a highly predictive serological test for HIV lymphoma.