Downregulated long non-coding RNA CLMAT3 promotes the proliferation of colorectal cancer cells by targeting regulators of the cell cycle pathway.
Downregulated long non-coding RNA CLMAT3 promotes the proliferation of colorectal cancer cells by targeting regulators of the cell cycle pathway.
复制标题
下调长非编码 RNA CLMAT3 通过靶向细胞周期途径的调节因子促进结直肠癌细胞的增殖。
DOI：
10.18632/oncotarget.10431
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发表时间：
2016-09-13
期刊：
影响因子：
--
通讯作者：
Wei Y
中科院分区：
文献类型：
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作者：
Ye LC;Chen T;Zhu DX;Lv SX;Qiu JJ;Xu J;Yuan FL;Wei Y
长链非编码RNA（lncRNA）-CLMAT 3的过表达与结直肠癌肝转移显著相关，并且是结直肠癌患者生存不良的独立预测因子。然而，由于对该基因在体外结肠直肠癌增殖中的作用知之甚少，我们研究了lncRNA-CLMAT 3在结肠直肠癌细胞增殖中的参与。在这项研究中，我们证明，lncRNA-CLMAT3表达显着增加，在结直肠癌细胞与正常肠粘膜细胞系相比，抑制lncRNA-CLMAT3抑制结直肠癌细胞在体外增殖。我们还发现，由于lncRNA-CLMAT3敲低导致的结直肠癌细胞增殖减少与G0/G1细胞周期阻滞诱导和凋亡增强相关。此外，lncRNA-CLMAT3敲低增强Cdh 1表达，并通过增加Skp 2蛋白泛素化导致p27 Kip积累。综上所述，我们的研究结果表明，减少lncRNA-CLMAT 3通过影响细胞周期组分来抑制结直肠癌细胞增殖。
Over-expression of long non-coding RNA (lncRNA)-CLMAT3 is significantly associated with colorectal liver metastasis and is an independent predictor of poor survival for colorectal cancer patients. However, as little is known regarding the role of this gene in the proliferation of colorectal cancer in vitro, we investigated the involvement of lncRNA-CLMAT3 in colorectal cancer cell proliferation. In this study, we demonstrate that lncRNA-CLMAT3 expression was significantly increased in colorectal cancer cells compared with a normal intestinal mucous cell line and that inhibition of lncRNA-CLMAT3 suppressed colorectal cancer cell proliferation in vitro. We also found that this reduced colorectal cancer cell proliferation due to lncRNA-CLMAT3 knockdown is associated with G0/G1 cell-cycle arrest induction and apoptosis enhancement. Furthermore, lncRNA-CLMAT3 knockdown enhanced Cdh1 expression and resulted in p27Kip accumulation via increased Skp2 protein ubiquitination. Taken together, our findings suggest that reducing lncRNA-CLMAT3 inhibits colorectal cancer cell proliferation by affecting cell cycle components.