Antidiabetic effects of Olea europaea L. leaves in diabetic rats induced by high-fat diet and low-dose streptozotocin
Antidiabetic effects of Olea europaea L. leaves in diabetic rats induced by high-fat diet and low-dose streptozotocin
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DOI：
10.1016/j.jep.2019.02.001
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发表时间：
2019-05-10
影响因子：
5.4
通讯作者：
Bauermann, Liliane de Freitas
中科院分区：
文献类型：
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作者：
Guex, Camille Gaube;Reginato, Fernanda Ziegler;Bauermann, Liliane de Freitas
民族药理学相关性：糖尿病（DM）是一种以高血糖、胰岛素抵抗和血脂异常为特征的代谢性疾病。它在世界范围内广泛发生，影响数百万人，并可能导致严重的并发症。橄榄（Olea europaea L.）具有重要的药理作用，包括抗炎、抗氧化和降血糖活性。橄榄叶是传统医学中用于治疗糖尿病和高血压的药物。研究目的：评价橄榄叶乙醇提取物（EEOL）对高脂饮食和低剂量链脲佐菌素（STZ）诱导的糖尿病大鼠代谢的影响。材料和方法：雄性Wistar大鼠被给予正常饲料或高脂肪饮食（70%标准实验室饲料，15%蔗糖，10%猪油和5%蛋黄粉）持续四周，随后施用STZ（35 mg/kg，经由ip）。空腹血糖水平高于200 mg/dL的动物视为糖尿病动物。将动物分为5组，在10周内通过经口灌胃给予乙醇（10 mL/kg）、二甲双胍（250 mg/kg）或200和400 mg/kg剂量的EEOL。血液样本用于测量血液学和生化参数，并取出肾脏进行后验分析。每周记录体重。结果：与对照组相比，在用乙醇和EEOL处理的糖尿病动物中观察到体重显著降低。此外，EEOL治疗的动物表现出改善葡萄糖水平和炎症和代谢标志物的水平相比，糖尿病animals.Conclusions：结果表明橄榄叶的潜在抗糖尿病活性，但需要更多的研究来验证临床效果。
Ethnopharmacological relevance: Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, insulin resistance, and dyslipidemia. It has broad occurrence worldwide, affecting millions of people, and can cause serious complications. The olive (Olea europaea L.) has important pharmacological functions, including anti-inflammatory, antioxidant, and hypoglycemic activities. Olive leaves are used in traditional medicine for diabetes and hypertension.Aim of the study: To evaluate the effect of the ethanolic extract of olive leaves (EEOL) on the metabolism of rats with diabetes induced by a high-fat diet and low dose of streptozotocin (STZ).Materials and methods: Male Wistar rats were either given normal feed or a high-fat diet (70% standard laboratory feed, 15% sucrose, 10% lard and 5% yolk powder) for four weeks, followed by administration of STZ (35 mg/kg, via ip). Animals with fasting glucose levels above 200 mg/dL were considered diabetic. Animals were divided into 5 groups, which received ethanol (10 mL/kg), metformin (250 mg/kg), or EEOL at doses of 200 and 400 mg/kg during 10 weeks by oral gavage. Blood samples were used to measure hematological and biochemical parameters, and kidneys were removed for posterior analysis. Body weight was recorded weekly.Results: A significant decrease in body weight was observed among diabetic animals treated with ethanol and EEOL compared to the control group. Moreover, animals treated with EEOL showed an improvement in glucose levels and in levels of inflammatory and metabolic markers when compared to diabetic animals.Conclusions: The results indicate a potential anti-diabetic activity of olive leaves, however more studies are needed to validate clinical effects.