Accumulation and biosynthesis of hydroxyl-α-sanshool in varieties of Zanthoxylum bungeanum Maxim. by HPLC-fingerprint and transcriptome analyses
Accumulation and biosynthesis of hydroxyl-α-sanshool in varieties of Zanthoxylum bungeanum Maxim. by HPLC-fingerprint and transcriptome analyses
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花椒品种羟基-α-山椒素的积累和生物合成。
DOI：
10.1016/j.indcrop.2019.111998
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发表时间：
2020-03-01
影响因子：
5.9
通讯作者：
Wang, Dongmei
中科院分区：
文献类型：
--
作者：
Wu, Zhaochen;Wang, Wei;Wang, Dongmei
花椒（Z.由于其烷基酰胺化合物（其中羟基-α-山椒醇（hydroxyl-alpha-sanshool）的含量最高），因此其药物性质和辛辣味道是众所周知的。对12个Z.采用反相高效液相色谱法（RP-HPLC）、HPLC指纹图谱和化学计量学方法对采自普通园林的花椒药材进行了评价。结果表明，油花椒（S12）果皮中总黄酮含量最高（46.936 ± 0.122 mg/g），河豚花椒（S2）果皮中总黄酮含量最低（2.235 ± 0.077 mg/g）。12个品种可分为4个类群，其相似性得分在0.424 ~ 1之间。进一步证明羟基-α-山椒醇是Z.臭牡丹为了探讨羟基-α-山椒醇引起这些差异的分子机制，从3个品种中鉴定了6656个差异表达基因（DEG（S2、S3、奉贤大红袍和S12），并且通过转录组分析确定羟基-α-山椒醇表达存在显著差异，包括10个与不饱和脂肪酸生物合成相关的DEG和9个与缬氨酸生物合成相关的DEG，可能参与羟基-α-山椒醇的生物合成。对功能注释后筛选出的6个DEG进行qRT-PCR（quantitative real-time-polymerase chain reaction）分析，结果与转录组测序结果一致，表明这些DEG可能是参与羟基-α-山椒醇生物合成的关键基因。本研究首次为玉米中烷基酰胺类化合物的定向生物合成、关键功能基因的筛选和品质育种提供了新的思路。bungeanum进一步研究。
Zanthoxylum bungeanum Maxim. (Z. bungeanum) is well known for its pharmaceutical properties and pungent taste due to its alkylamide compounds, among which hydroxyl-alpha-sanshool has the highest content. The differences in the hydroxyl-alpha-sanshool profiles among 12 varieties of Z. bungeanum collected from a common garden were evaluated by RP-HPLC (reversed-phase high-performance liquid chromatography), HPLC fingerprint and chemometric analyses. The results showed that the peel of You Huajiao (S12) exhibited the highest content (46.936 +/- 0.122 mg/g) and Fugu Huajiao (S2) exhibited the lowest content (2.235 +/- 0.077 mg/g). The twelve varieties were divided into four groups, and their scores of similarity were between 0.424 and 1. Moreover, hydroxyl-alpha-sanshool was further proven to be the key compound in Z. bungeanum. To explore the molecular mechanisms of hydroxyl-alpha-sanshool causing these differences, 6656 differentially expressed genes (DEGs) were identified from three varieties(S2, S3 Fengxian Dahongpao and S12)and showed a significant difference in hydroxyl-alpha-sanshool expression, as determined by transcriptome analysis, including 10 DEGs related to the biosynthesis of unsaturated fatty acids and 9 DEGs related to valine biosynthesis, which were probably involved in hydroxyl-alpha-sanshool biosynthesis. The results of qRT-PCR (quantitative real-time-polymerase chain reaction) analysis for 6 DEGs selected after functional annotation were equivalent to those detected by transcriptome sequencing, implying that these DEGs may be the key genes involved in hydroxyl-alpha-sanshool biosynthesis. The present comprehensive analysis provides, for the first time, insight into the directional biosynthesis of alkylamide compounds, the selection of key functional genes and quality breeding in Z. bungeanum for further study.