Sizing up metatranscriptomics
Sizing up metatranscriptomics
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DOI：
10.1038/ismej.2012.94
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发表时间：
2013-02-01
期刊：
影响因子：
11
通讯作者：
Hopkinson, Brian M.
中科院分区：
文献类型：
--
作者：
Moran, Mary Ann;Satinsky, Brandon;Hopkinson, Brian M.
在沿海海水中，一个典型的海洋细菌细胞只含有大约200个mRNA分子，每个mRNA分子在降解前只能持续几分钟。这样一个令人惊讶的小而动态的细胞mRNA库对于理解细菌对环境信号的反应以及我们测量这些反应的能力具有重要意义。从这个角度来看，我们审查了环境细菌中的转录动力学的可用数据，然后考虑微生物群落的功能宏基因组研究的小而短暂的mRNA库存的后果。
A typical marine bacterial cell in coastal seawater contains only similar to 200 molecules of mRNA, each of which lasts only a few minutes before being degraded. Such a surprisingly small and dynamic cellular mRNA reservoir has important implications for understanding the bacterium's responses to environmental signals, as well as for our ability to measure those responses. In this perspective, we review the available data on transcript dynamics in environmental bacteria, and then consider the consequences of a small and transient mRNA inventory for functional metagenomic studies of microbial communities.