Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation
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DOI：
10.1126/science.284.5421.1811
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发表时间：
1999-06-11
期刊：
影响因子：
56.9
通讯作者：
Malinow, R
中科院分区：
文献类型：
--
作者：
Shi, SH;Hayashi, Y;Malinow, R
为了监测活体神经元中α -氨基-3-羟基-5-甲基-4-异唑丙酸（AMPA）受体分布的变化，用绿色荧光蛋白（GFP）标记AMPA受体亚基GluR1，该蛋白（GluR1-GFP）具有功能性，在海马CA1神经元中短暂表达，在双光子激光扫描显微镜或电子显微镜下可见，GluR1-GFP大部分在细胞内，模拟内源性GluR1分布。强直性突触刺激诱导标记受体快速传递到树突棘和树突簇。这些突触后转运事件需要突触n -甲基-d -天冬氨酸（NMDA）受体激活，并可能有助于在长期增强和活性依赖的突触成熟过程中观察到AMPA受体介导的增强传递。
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP), This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons, in dendrites visualized with two-photon Laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediated transmission observed during long-term potentiation and activity-dependent synaptic maturation.