Flash fluorescence induction:: a novel method to study regulation of Photosystem II
Flash fluorescence induction:: a novel method to study regulation of Photosystem II
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DOI：
10.1016/s1011-1344(99)00032-9
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发表时间：
1999-02-01
影响因子：
5.4
通讯作者：
Kaftan, D
中科院分区：
文献类型：
--
作者：
Nedbal, L;Trtílek, M;Kaftan, D
我们证明，发光二极管阵列可用于产生单周转闪光，使绿藻中的 Q(A) 减少饱和。双调制荧光计的快速版本可以测量单周转饱和闪光期间的感应（闪光荧光感应）。该方法可以测量光系统 II 的有效天线尺寸、天线异质性和连接性，而不会用阻止 Q(A)(-) 再氧化的除草剂毒害生物体。使用同一仪器，我们还通过与含氧复合物中闪光诱导的 S 态进展相关的荧光瞬态测量了光系统 II 的功能异质性。 (C) 1999 Elsevier Science S.A. 保留所有权利。
We demonstrate that arrays of light-emitting diodes can be used to generate single-turnover flashes of light that saturate Q(A) reduction in green algae. The fast version of a double-modulation fluorometer can measure induction during a single-turnover saturating flash (flash fluorescence induction). The method allows the effective antenna size, antenna heterogeneity and connectivity of Photosystem II to be measured without poisoning the organism with herbicide that blocks Q(A)(-) reoxidation. Using the same instrument, we have also measured the functional heterogeneity of Photosystem II by the fluorescence transient related to the flash-induced advancement of S-states in the oxygen-envolving complex. (C) 1999 Elsevier Science S.A. All rights reserved.