Distribution of smooth muscle cells and macrophages expressing scavenger receptor BI/II in atherosclerosis.
Distribution of smooth muscle cells and macrophages expressing scavenger receptor BI/II in atherosclerosis.
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DOI：
10.5551/jat.1941
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发表时间：
2009
影响因子：
4.4
通讯作者：
Y. Ishikawa;Masayo Kimura-Matsumoto;M. Murakami;Kei Yamamoto;Y. Akasaka;M. Uzuki;Yuri Yuri-Yuri;Naomi Inomata;Tomoko Yokoo;T. Ishii
中科院分区：
文献类型：
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作者：
Y. Ishikawa;Masayo Kimura-Matsumoto;M. Murakami;Kei Yamamoto;Y. Akasaka;M. Uzuki;Yuri Yuri-Yuri;Naomi Inomata;Tomoko Yokoo;T. Ishii
目的I型和II型清道夫受体（Scavenger receptor type I and II，SRBI/II）通过与脂蛋白的相互作用及其在巨噬细胞中的表达，具有致动脉粥样硬化和抗动脉粥样硬化的双重作用，但SRBI/II阳性巨噬细胞和平滑肌细胞（smooth muscle cell，SMCs）在人主动脉粥样硬化内膜中的分布、密度及其与脂代谢相关蛋白的关系尚不清楚。方法采用SRBI/BII和平滑肌肌动蛋白或巨噬细胞特异性抗体对尸检主动脉组织进行免疫组化双重染色。测量内膜损伤处SRBI/BII阳性SMC和巨噬细胞的密度。他们还免疫染色抗体对四个载脂蛋白，四个磷脂酶A2，和CETP。结果SRBI/II在内膜病变中的巨噬细胞和平滑肌细胞均有表达。随着动脉粥样硬化的进展，内膜病变中SRBI/II阳性SMC的密度显著降低，而SRBI/II阳性巨噬细胞的密度显著增加。此外，功能蛋白，如载脂蛋白，分泌型磷脂酶A2，和CETP，分布在内膜基质周围SRBI/II阳性细胞在所有病变类型。结论动脉粥样硬化早期SMC主要通过SRBI/II表达参与脂质代谢，晚期SMC主要通过SRBI/II表达参与脂质代谢。
AIM Scavenger receptors type I and II (SRBI/II) have dual roles in both atherogenic and antiatherogenic functions through interactions with lipoproteins and their expression in macrophages; how-ever, the distribution and density of SRBI/II-positive macrophages and smooth muscle cells (SMCs) as well as their association with lipid metabolism-related proteins in atherosclerotic intima of the human aorta remain unclear. METHODS Autopsied aortic tissues were double-immunostained with SRBI/BII and smooth muscle actin or macrophage-specific antibodies. The density of SRBI/BII-positive SMCs and macrophages in intimal lesion was measured. They were also immunostained with antibodies against four apolipoproteins, four phospholipase A2s, and CETP. RESULTS SRBI/II was expressed in both macrophages and SMCs distributed in various intimal lesions. The density of SRBI/II-positive SMCs in intimal lesions significantly decreased with the advance of atherosclerosis, whereas the density of SRBI/II-positive macrophages significantly increased with atherosclerotic development. In addition, functional proteins, such as apolipoproteins, secretory phospholipase A2s, and CETP, were distributed in the intimal stroma around SRBI/II-positive cells in all lesion types. CONCLUSION The results indicated that SMCs are involved in lipid metabolism via SRBI/II expression mainly in the early stages of atherosclerosis evolution, and that SRBI/II-positive macrophages are mainly involved in advanced stages.