Direct visualization of mRNA colocalization with mitochondria in living cells using molecular beacons
Direct visualization of mRNA colocalization with mitochondria in living cells using molecular beacons
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DOI：
10.1117/1.2011402
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发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Bao, G
中科院分区：
文献类型：
--
作者：
Santangelo, PJ;Nitin, N;Bao, G
mRNA的细胞内定位和特定的细胞器关联可以反映mRNA的基本功能、阶段和稳定性。我们报告直接可视化的K-ras和甘油醛3-磷酸脱氢酶（GAPDH）mRNA的亚细胞定位在活HDF细胞使用分子信标与膜透化和肽为基础的交付。出乎意料的是，我们发现K-ras和GAPDH mRNA与线粒体共定位。进行了广泛的对照研究，包括使用荧光原位杂交（FISH），阴性对照信标，和共定位的28 S核糖体RNA与粗面内质网（ER）的检测，这表明在我们的研究中观察到的mRNA定位和共定位模式是真实的和具体的。我们的观察揭示了有趣的亚细胞协会的mRNA与细胞器，如线粒体，这可能会提供新的见解的运输，动力学和功能的mRNA和mRNA-蛋白质相互作用。(C)2005年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
The intracellular localization and specific organelle association of mRNA may reflect essential functions, stages, and stability of mRNA. We report the direct visualization of subcellular localization of K-ras and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs in live HDF cells using molecular beacons together with membrane-permeabilization and peptide-based delivery. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive control studies are performed, including the use of fluorescence in-situ hybridization (FISH), negative-control beacons, and the detection of colocalization of 28S ribosomal RNA with the rough endoplasmic reticulum (ER), suggesting that the mRNA localization and colocalization patterns observed in our study are true and specific. Our observation reveals intriguing subcellular associations of mRNA with organelles such as mitochondria, which may provide new insight into the transport, dynamics, and functions of mRNA and mRNA-protein interactions. (C) 2005 Society of Photo-optical Instrumentation Engineers.