The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit
The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit
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DOI：
10.1016/j.postharvbio.2006.06.008
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发表时间：
2006-11-01
影响因子：
7
通讯作者：
Tonutti, Pietro
中科院分区：
文献类型：
--
作者：
Dal Cin, Valeriano;Rizzini, Fabio Massimo;Tonutti, Pietro
1-甲基环丙烯 (1-MCP) 是一种乙烯受体结合位点拮抗剂，其应用效果因基因型和成熟生理等多种因素而异。将桃子 (Prunus persica L. Batsch cv. 'Summer Rich') 和苹果 (Malus x Domestica L. Borkh cv. 'Golden Delicious') 果实与 1-MCP (1 mu L l(-1)) 在 20°C 下孵育 24 小时，并与 ACC 合酶、ACC 氧化酶、ETR1、ERS1 和 CTR1 一起培养呼吸速率、乙烯产量和果实硬度基因表达 在整个治疗后阶段对模式进行评估。 1-MCP 被证实可有效延迟苹果成熟，而在桃子中仅观察到该化学物质的有限作用。 1-MCP 在苹果中诱导乙烯生物合成和 ACS 基因表达的显着抑制，而在桃子中，两个对照（在空气中和在没有 1-MCP 的密封罐中）和处理过的水果之间没有观察到明显差异。在苹果中，Md-ETR1 和 Md-ERS1 基因表达从处理结束开始就被 1-MCP 下调，而 Md-CTR1 在后期似乎受到该化学物质的负面影响。在 1-MCP 处理的桃子中，Pp-ETR1、Pp-ERS1 和 Pp-CTR1 基因的转录似乎不受影响。保存在空气中的对照水果与密封在不含 1-MCP 的密封罐中的水果之间受体转录水平的差异可能是由于在成熟桃子孵化后迅速积累的 CO2 的影响。结果支持这样的假设：桃子和苹果对 1-MCP 应用的不同行为可能与乙烯受体的比率、表达模式和/或周转率方面的差异有关。 (C) 2006 Elsevier B.V. 保留所有权利。
1-Methylcyclopropene (1-MCP) is an antagonist of ethylene for receptor binding sites and the effects of its application differ in relation to a number of factors including genotype and ripening physiology. Peach (Prunus persica L. Batsch cv. 'Summer Rich') and apple (Malus x domestica L. Borkh cv. 'Golden Delicious') fruits were incubated with 1-MCP (1 mu L l(-1)) for 24 h at 20 degrees C and respiration rate, ethylene production and fruit firmness, together with ACC synthase, ACC oxidase, ETR1, ERS1, and CTR1 gene expression patterns were assessed throughout the post-treatment phase. 1-MCP was confirmed to be effective in delaying ripening in apples while in peaches only a limited effect of the chemical was observed. A dramatic inhibition of ethylene biosynthesis and ACS gene expression was induced by 1-MCP in apples whereas no marked difference was observed in peaches between the two controls (in air and in sealed jars without 1-MCP) and the treated fruit. In apples, Md-ETR1 and Md-ERS1 gene expression was down-regulated by 1-MCP starting from the end of the treatment, while Md-CTR1 appeared negatively affected by the chemical at a later stage. Transcription of Pp-ETR1, Pp-ERS1 and Pp-CTR1 genes appeared unaffected in 1-MCP treated peaches. Differences in receptor transcript levels between control fruit maintained in air and those enclosed in sealed jars without 1-MCP may be due to an effect of CO2 that rapidly accumulates following incubation of ripening peaches. Results support the hypothesis that the different behaviour of peaches and apples in response to 1-MCP application might be related to differences in terms of ratio, expression patterns and/or turn-over of the ethylene receptors. (C) 2006 Elsevier B.V. All rights reserved.