Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae.
Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae.
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DOI：
10.1002/yea.320010202
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发表时间：
1985-12-01
期刊：
影响因子：
--
通讯作者：
Bostian, K A
中科院分区：
文献类型：
--
作者：
Parent, S A;Fenimore, C M;Bostian, K A
在过去的七年里，我们对酿酒糖酵母中许多细胞和分子现象的理解大大增加，这主要是由于新的酵母重组DNA技术所提供的经典遗传学和分子方法的结合。导致酵母DNA转化系统开发的开创性研究，导致这些技术的改进和精细化的早期努力，以及许多新质粒的加入，已经导致设计用于研究这种真核生物的分子生物学的复杂系统阵列。
Our understanding of many cellular and molecular phenomena in the yeast Succharomyces cerevisiae has increased dramatically over the past seven years due largely to the combination of classical genetics and molecular approaches afforded by the new yeast recombinant DNA technology. The pioneering research leading to development of a yeast DNA transformation system,'-3 early efforts leading to the refinement and elaboration of these techniques4-lo and the addition of many new plasmids have resulted in a sophisticated array of systems designed to study the molecular biology of this eucaryote.