Divergent Effect of Dezocine, Morphine and Sufentanil on Intestinal Motor Function in Rats.
Divergent Effect of Dezocine, Morphine and Sufentanil on Intestinal Motor Function in Rats.
复制标题
地佐辛、吗啡和舒芬太尼对大鼠肠运动功能的不同影响。
DOI：
10.7150/ijms.12616
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发表时间：
2015
影响因子：
3.6
通讯作者：
Wen D
中科院分区：
文献类型：
--
作者：
Bian X;Zhou R;Yang Y;Li P;Hang Y;Hu Y;Yang L;Wen D
背景：阿片类药物诱导的肠道功能障碍是术前使用吗啡、芬太尼及其衍生物最常见的副作用。然而，地佐辛对肠道蠕动的影响鲜有报道。本研究旨在探讨地佐辛、吗啡和舒芬太尼对大鼠肠道平滑肌收缩及推进功能的影响。
方法：将不同浓度的地佐辛、吗啡和舒芬太尼与分离的大鼠小肠平滑肌孵育后，使用张力换能器测量其收缩张力和频率。腹腔注射吗啡、舒芬太尼和地佐辛30分钟后，测定大鼠肠道内亚甲蓝的推进速率。计算与基线水平相比收缩张力和收缩频率的变化百分比，以评估肌肉收缩情况。亚甲蓝推进速率按亚甲蓝在肠道内移动距离占小肠长度的百分比计算。
结果：高剂量的吗啡和舒芬太尼显著增加分离的小肠平滑肌收缩张力。在三个测试剂量下，收缩频率均无显著变化。将地佐辛剂量从1.7mg/L增加到10.2mg/L，收缩张力和收缩频率均无变化。与对照组（57.1%）相比，吗啡、舒芬太尼和地佐辛处理后，肠道内亚甲蓝的推进速率显著降低（分别为45.6%、43.7%和42.1%），但三个药物组之间差异不显著。
结论：吗啡和舒芬太尼可能剂量依赖性地增加分离的大鼠小肠平滑肌的收缩张力和收缩能力，而地佐辛对小肠平滑肌收缩无显著影响。然而，所有这些阿片类药物都可能损害小肠推进功能。
Background: Opioid induced bowel dysfunction is the most common side effect of preoperatively administrated morphine, fentanyl and its derivative. However, the influence of dezocine on intestinal mobility is rarely reported. This study was designed to investigate the effects of dezocine, morphine and sufentanil on both intestinal smooth muscle contraction and propulsion in rats. Methods: Contractile tension and frequency of isolated rat small intestine smooth muscle were measured using tension transducer after incubation with different concentrations of dezocine, morphine and sufentanil. The propulsive rate of methylene blue in rat intestinal tract was measured 30 minutes after intraperitoneal injection of morphine, sufentanil and dezocine. Percent of change in contractile tension and contraction frequency compared to baseline level were calculated to evaluate muscle contraction. Propulsive rate of methylene blue was calculated as the percentage of methylene blue moving distance in intestinal tract compared to the length of the small intestine. Results: Morphine and sufentanil significantly increased the contractile tension of isolated small intestine smooth muscle at high doses. The contraction frequency did not change significantly among the 3 tested doses. Increasing the dose of dezocine from 1.7 mg.L-1 to 10.2 mg.L-1 did not change either the contractile tension or the contraction frequency. The propulsive rate of methylene blue in intestinal tract was significantly decreased after the treatment with morphine, sufentanil and dezocine (45.6%, 43.7%, and 42.1% respectively) compared to control group(57.1%), while the difference among the 3 drug groups were not significant. Conclusion: Morphine and sufentanil may dose dependently increase the contractile tension and contraction ability of isolated rat small intestine smooth muscle, while dezocine has no significant effect on intestine smooth muscle contraction. However, all these opioids might impair small intestinal propulsion.