Gold nanoparticle triggered chemilumineseence between luminol and AgNO3
Gold nanoparticle triggered chemilumineseence between luminol and AgNO3
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金纳米颗粒引发鲁米诺和 AgNO3 之间的化学发光
DOI：
10.1021/jp800749y
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发表时间：
2008-07-31
影响因子：
3.7
通讯作者：
Liu, Li-Jia
中科院分区：
文献类型：
--
作者：
Cui, Hua;Guo, Ji-Zhao;Liu, Li-Jia
研究发现，鲁米诺在胶体金存在下可以与AgNO3反应，产生425 nm的化学发光（CL）。紫外可见光谱和X射线光电子能谱表明，在CL反应中，AgNO3被鲁米诺还原为Ag，并覆盖在金纳米粒子的表面，形成Au/Ag核/壳纳米粒子。通过 CL 光谱鉴定该发光体为 3-氨基邻苯二甲酸酯。还研究了不同成分的金/银核/壳纳米粒子对鲁米诺-AgNO3 体系和化学发光动力学的影响。在此基础上，提出了涉及催化的CL反应机理。当将Au胶体注入鲁米诺和AgNO3的混合物中时，AgNO3在纳米金的催化下与鲁米诺快速反应，产生Ag和鲁米诺自由基，与溶解的氧反应，并伴随发光；然后，随着Ag原子在Au颗粒表面的瞬间沉积，催化活性下降，导致CL强度急剧下降和核/壳纳米颗粒的缓慢生长。新型化学发光体系具有背景低、稳定性好的优点，并且金纳米颗粒具有优异的生物相容性。它未来可能会在免疫测定和 DNA 检测中得到应用。
It was found that luminol could react with AgNO3 in the presence of gold colloid to generate chemiluminescence (CL) at 425 nm. UV-visible spectra and X-ray photoelectron spectra showed that AgNO3 was reduced by luminol to Ag in the CL reaction, which covered on the surface of gold nanoparticles to form Au/Ag core/shell nanoparticles. The luminophor was identified by the CL spectrum as 3-aminophthalate. The effects of Au/Ag core/shell nanoparticles with various compositions on the luminol-AgNO3 system and the CL kinetics were also studied. On this basis, a CL reaction mechanism involving catalysis is proposed. When Au colloid was injected into the mixture of luminol and AgNO3, AgNO3 reacted rapidly with luminol under the catalysis of gold nanoparticles to produce Ag and luminol radicals, which reacted with the dissolved oxygen, accompanying a light emission; then, with instant deposition of Ag atoms on the surface of Au particles, the catalytic activity declined, leading to a sharp decrease in CL intensity and a slow growth of core/shell nanoparticles. The new CL system has the advantages of low background and good stability, and gold nanoparticles have excellent biocompatible property. It may find future applications in immunoassay and DNA detection.