Influence of Platycodonis Radix and Achyranthis Bidentatae Radix in Xuefu Zhuyu Tang on Tissue Distribution of Paeoniflorin
Influence of Platycodonis Radix and Achyranthis Bidentatae Radix in Xuefu Zhuyu Tang on Tissue Distribution of Paeoniflorin
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发表时间：
2015
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通讯作者：
Huang We
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作者：
Huang We
目的：比较桔梗、牛膝不同配伍对芍药苷在正常小鼠和血瘀小鼠体内组织分布的影响，初步探讨桔梗、牛膝的导向作用。方法：正常组和血瘀组小鼠灌胃血府逐瘀汤，缺桔梗或牛膝方，30 min后处死小鼠，取全血，取脑、心、肝、脾、肺、肾。采用高效液相色谱法，以京尼平苷为内标，以乙腈-水（16∶ 84）为移动的流动相，检测波长为230 nm。测试结果：正常小鼠灌胃血府逐瘀汤全方、单缺桔梗方、单缺牛膝方、单缺桔梗和牛膝方后，肺组织中芍药苷含量均为0。010 2.0。005 8.0。0910.0。037 3 μg·g-1，肾组织中为0。122 2.0 052 0.0。144 0.0。0650 μg·g-1，但芍药苷在脑、心、肝、脾中未被抑制。血府逐瘀汤全方、单缺桔梗方灌胃给药后，小鼠肺组织中芍药苷含量分别为0。076 4.0。042 0 μg·g-1，肾组织0. 335,0. 210 μg·g-1，但在脑、心、肝、脾中未检出。血府逐瘀汤单独灌胃给药后，小鼠肺组织中芍药苷含量为0。274 7.0 019 7 μg·g-1，肾脏中含量为0. 467 1.0。149 0μg·g-1，在脑、心、肝、脾中几乎未检出。结论：以血府逐瘀汤缺桔梗、牛膝方为对照，桔梗能增加正常小鼠肺、肾中芍药苷的含量，桔梗能增加血瘀小鼠肺中芍药苷的含量，且增加作用较明显，牛膝能降低正常小鼠肺、肾中芍药苷的含量，能增加血瘀模型小鼠肺、肾组织中芍药苷的含量，并能使血瘀模型小鼠心、肝、脾组织中芍药苷分布均匀。本研究证实桔梗和怀牛膝具有主导作用。桔梗和牛膝的主要部位不单一。
Objective: To compare effect of different compatibility of Platycodonis Radix and Achyranthis Bidentatae Radix on tissue distribution of paeoniflorin in normal and blood stasis mice,preliminary study leading action of Platycodonis Radix and Achyranthis Bidentatae Radix. Method: Normal and blood stasis mice were gavaged with Xuefu Zhuyu Tang,lacked Platycodonis Radix or Achyranthis Bidentatae Radix parties.Mice were killed after 30 minutes,whole blood collected,the brain,heart,liver,spleen,lung and kidney were removed. HPLC was employed to determine the content of paeoniflorin with mobile phase of acetonitrile-water( 16∶ 84),detection wavelength at 230 nm and geniposide as internal standard. Result: After normal mice being gavaged with Xuefu Zhuyu Tang full party, single lacked Platycodonis Radix party, single lacked Achyranthis Bidentatae Radix party, lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, the content of paeoniflorin in lung were 0. 010 2,0. 005 8,0. 091 0,0. 037 3 μg·g-1,those in kidney were 0. 122 2,0. 052 0,0. 144 0,0. 065 0 μg·g-1,but paeoniflorin were unchecked in the brain,heart,liver and spleen. After blood stasis mice being gavaged with Xuefu Zhuyu Tang full party,single lacked Platycodonis Radix party,the content of paeoniflorin in lung were 0. 076 4,0. 042 0 μg·g-1,those in kidney were 0. 335,0. 210 μg·g-1,but it was unchecked in the brain,heart,liver and spleen. After blood stasis mice being gavaged with Xuefu Zhuyu Tang single lacked Achyranthis Bidentatae Radix party,lacked Platycodonis Radix and Achyranthis Bidentatae Radix party,the content of paeoniflorin in lung were 0. 274 7,0. 019 7 μg·g-1,those in kidney were 0. 467 1,0. 149 0μg·g-1,it almost were unchecked in the brain,heart,liver and spleen. Conclusion: Making comparison based on Xuefu Zhuyu Tang lacked Platycodonis Radix and Achyranthis Bidentatae Radix party,Platycodonis Radix can increase the content of paeoniflorin in the lung and kidney in normal mice, Platycodonis Radix can more significantly increase the content of paeoniflorin in the lung than that in the kidney in blood stasis mice.Achyranthis Bidentatae Radix can decrease the content of paeoniflorin in the lung and kidney in normal mice,it can increase the content of paeoniflorin in the lung and kidney in blood stasis mice,it also can make distribution of paeoniflorin in the heart, liver, spleen in blood stasis mice. This study confirms Platycodonis Radix and Achyranthis Bidentatae Radix have effect of leading action. Leading sites of Platycodonis Radix and Achyranthis Bidentatae Radix are not single.