Microfluidic chip for automated screening of carbon dioxide conditions for microalgal cell growth
Microfluidic chip for automated screening of carbon dioxide conditions for microalgal cell growth
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DOI：
10.1063/1.5012508
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发表时间：
2017-11-01
期刊：
影响因子：
3.2
通讯作者：
Dong, Liang
中科院分区：
文献类型：
--
作者：
Xu, Zhen;Wang, Yingjun;Dong, Liang
本文报道了一种能够筛选微藻细胞生长所需二氧化碳（CO2）条件的微流控装置。该装置主要由微流控细胞培养（MCC）单元、气体浓度梯度发生器（CGG）和细胞在线生长光学测量单元组成。MCC单元的结构是在顶层有多个充满水的细胞培养通道，在底层有多个CO2流动通道，在两个通道层之间夹有一个商业疏水气体半透膜。CGG装置提供不同的CO2浓度，以支持培养通道中微藻的光合作用。将商业气体半透膜集成到细胞培养装置中，无需使用传统的搅拌辅助对流方法，就可以在培养基中快速传输质量和均匀分布CO2，因为CO2从气体流动通道扩散到培养通道在小长度范围内是快速的。此外，通过光学测量单元实现了微藻细胞生长的自动在线监测，该光学测量单元能够检测通过培养通道中细胞培养物传递的光强度的变化。微流控装置还允许一个简单的灰度分析方法来量化细胞生长。通过在不同的低或极低的二氧化碳水平（低于标称环境二氧化碳浓度）下培养莱茵衣藻细胞，验证了系统的实用性。AIP出版社出版。
This paper reports on a microfluidic device capable of screening carbon dioxide (CO2) conditions for microalgal cell growth. The device mainly consists of a microfluidic cell culture (MCC) unit, a gas concentration gradient generator (CGG), and an in-line cell growth optical measurement unit. The MCC unit is structured with multiple aqueous-filled cell culture channels at the top layer, multiple CO2 flow channels at the bottom layer, and a commercial hydrophobic gas semipermeable membrane sandwiched between the two channel layers. The CGG unit provides different CO2 concentrations to support photosynthesis of microalgae in the culture channels. The integration of the commercial gas semipermeable membrane into the cell culture device allows rapid mass transport and uniform distribution of CO2 inside the culture medium without using conventional agitation-assisted convection methods, because the diffusion of CO2 from the gas flow channels to the culture channels is fast over a small length scale. In addition, automated in-line monitoring of microalgal cell growth is realized via the optical measurement unit that is able to detect changes in the light intensity transmitted through the cell culture in the culture channels. The microfluidic device also allows a simple grayscale analysis method to quantify the cell growth. The utility of the system is validated by growing Chlamydomonas reinhardtii cells under different low or very-low CO2 levels below the nominal ambient CO2 concentration. Published by AIP Publishing.