Effects of cholecystokinin, caerulein and pentagastrin on electrical behavior of myenteric neurons.
Effects of cholecystokinin, caerulein and pentagastrin on electrical behavior of myenteric neurons.
复制标题
胆囊收缩素、雨伞素和五肽胃泌素对肌间神经元电行为的影响。
DOI：
10.1016/0014-2999(85)90161-x
复制
发表时间：
1985
影响因子：
5
通讯作者：
J. Wood
中科院分区：
文献类型：
--
作者：
P. Nemeth;D. Zafirov;J. Wood
采用细胞内法记录豚鼠离体回肠肌肠神经元的电行为。将1 ~ 100 μM的缩胆素-八肽（CCK-8）和1 ~ 10 μM的小檗碱（caerulein）通过压力微射注入神经元。CCK-8 （0.01 ~ 1 μM）和caerulein （0.01 ~ 0.1 μM）也加入了超熔液中。CCK-8，无论使用哪种方法，都诱发了细胞膜的长期去极化，这与输入电阻的增加、超极化后电位的抑制和兴奋性的增强有关。细胞内注入电流使细胞膜去极化过程中动作电位放电的概率增加，反映了兴奋性的增强。小毛蛋白引起的兴奋反应与CCK-8相同。两种药物的作用模拟了神经元中缓慢的突触兴奋。在另一组AH/ 2型神经元中，CCK-8引起了长时间的膜超极化和输入阻抗的降低，模拟了刺激引起的慢抑制性突触后电位。超极化反应从未被小毛豆蛋白引起。大约25%的神经元对CCK-8没有反应，70%的神经元对小毛蛋白没有反应。加1 μM的Pentagastrin对神经元的电行为没有影响。结果与胆囊收缩素的神经递质作用一致，胆囊收缩素可能在肠神经系统中具有兴奋性或抑制性。
Intracellular methods were used to record the electrical behavior of myenteric neurons from guinea-pig ileum in vitro. Cholecystokinin-octapeptide (CCK-8) at 1 to 100 μM and caerulein at 1 to 10 μM in the ejection pipettes were applied to the neurons by pressure microejection. CCK-8 (0.01 to 1 μM) and caerulein (0.01 to 0.1 μM) were applied also in the superfusion solution. CCK-8, applied by either method, evoked a long-lasting depolarization of the cell membranes that was associated with an increase in the input resistance, suppression of hyperpolarizing afterpotentials and enhanced excitability. The enhanced excitability was reflected by increased probability of action potential discharge during membrane depolarization by intracellularly injected current. Caerulein evoked the same excitatory responses as CCK-8. The actions of both agents simulated slow synaptic excitation in the neurons. In another group of AH/type 2 neurons, CCK-8 evoked a long-lasting membrane hyperpolarization and decreased input resistance that mimicked stimulus-evoked slow inhibitory postsynaptic potentials. Hyperpolarizing responses were never evoked by caerulein. About 25% of tested neurons failed to respond to CCK-8 and 70% did not respond to caerulein. Pentagastrin (1 μM in the superfusion solution) did not affect the electrical behavior of the neurons. The results are consistent with a neurotransmitter role for cholecystokinin that could be either excitatory of inhibitory in the enteric nervous system.
毛喉素模仿肌间神经元的缓慢突触兴奋。
影响因子：
5
作者：
Nemeth,PR;Zafirov,D;Wood,JD
通讯作者：
Wood,JD