Genetic diversity and evolution of the virulence plasmids encoding aerobactin and salmochelin in Klebsiella pneumoniae.
Genetic diversity and evolution of the virulence plasmids encoding aerobactin and salmochelin in Klebsiella pneumoniae.
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肺炎克雷伯菌编码需氧菌素和沙莫螯合素的毒力质粒的遗传多样性和进化
DOI：
10.1080/21505594.2021.1924019
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Jiang X
中科院分区：
文献类型：
--
作者：
Tian D;Wang M;Zhou Y;Hu D;Ou HY;Jiang X
摘要 高毒力肺炎克雷伯菌（hvKp）的毒力质粒有可能转移到耐药菌株或与其他质粒整合，促进威胁病原体的基因组进化。我们对公开的156个hvKp完整基因组序列以及来自中国的171个hvKp菌株的多区域临床队列进行了深入分析，为毒力质粒进化提供证据。在 ST23 和 ST11 肺炎克雷伯菌菌株中经常检测到毒力质粒。多重耐药hvKp（MDR-hvKp）在hvKp中占有很大比例，毒力和耐药质粒的共存可能是主要原因。毒力质粒通常具有多个复制子，其中 IncFIBK 最为普遍（84.6%）。我们在583个IncFIBK质粒中鉴定出49个IncFIBK等位基因，它们可分为Clades I、II和III。我们进一步观察到，接合型和非接合型毒力质粒可以通过 IncFIBK 遗传多样性来区分，并且 IncFIBK 亚型也可以间接表明接合型毒力质粒的嵌合偏好。在此基础上，我们开发了一个名为 KpVR 的开放访问网络工具，用于 IncFIBK 子类型分析。总之，IncFIBK毒力质粒的遗传多样性可用于追踪毒力质粒的进化，进一步防止MDR-hvKp菌株的出现。
ABSTRACT Virulence plasmids of hypervirulent Klebsiella pneumoniae (hvKp) have the potential to transfer to drug-resistant strains or integrate with other plasmids, facilitating the genome evolution of threatening pathogens. We conducted an in-depth analysis of the publicly available 156 complete genome sequences of hvKp together with a multi-region clinical cohort of 171 hvKp strains from China to provide evidence for the virulence plasmid evolution. Virulence plasmids were frequently detected in the ST23 and ST11 K. pneumoniae strains. Multidrug-resistant hvKp (MDR-hvKp) occupied a large proportion of hvKp, and the coexistence of virulence and resistance plasmids may be the major cause. Virulence plasmids commonly possessed multiple replicons, of which IncFIBK was the most prevalent (84.6%). We identified 49 IncFIBK alleles among 583 IncFIBK plasmids, and they could be divided into Clades I, II, and III. We further observed that conjugative and non-conjugative virulence plasmids could be distinguished by IncFIBK genetic diversity, and IncFIBK subtyping could also indirectly indicate a chimeric preference of conjugative virulence plasmids. On this basis, we developed an open-access web tool called KpVR for IncFIBK subtyping. In conclusion, the genetic diversity of IncFIBK virulence plasmids could be used for tracking the evolution of virulence plasmids, and further preventing the emergence of MDR-hvKp strains.
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影响因子：
11.8
作者：
Chen, Yahua;Marimuthu, Kalisvar;Gan, Yunn-Hwen
通讯作者：
Gan, Yunn-Hwen
blaKPC-2 在中国肺炎克雷伯菌 ST11 中的大流行与 IncFII 样质粒介导的水平转移有关
DOI：
10.1016/j.ijantimicag.2019.03.014
发表时间：
2019-08-01
影响因子：
10.8
作者：
Fu, Pan;Tang, Yu;Jiang, Xiaofei
通讯作者：
Jiang, Xiaofei
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
影响因子：
3.1
作者：
Russo, Thomas A.;Olson, Ruth;Gulick, Andrew M.
通讯作者：
Gulick, Andrew M.
影响因子：
3.7
作者：
Kaas RS;Leekitcharoenphon P;Aarestrup FM;Lund O
通讯作者：
Lund O