MicroRNA miR-181a correlates with morphological sub-class of acute myeloid leukaemia and the expression of its target genes in global genome-wide analysis
MicroRNA miR-181a correlates with morphological sub-class of acute myeloid leukaemia and the expression of its target genes in global genome-wide analysis
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DOI：
10.1038/sj.leu.2404605
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发表时间：
2007-05-01
期刊：
影响因子：
11.4
通讯作者：
Young, B. D.
中科院分区：
文献类型：
--
作者：
Debernardi, S.;Skoulakis, S.;Young, B. D.
microRNA（miRNAs）是一类短链的单链RNA，在基因调控中具有潜在的重要作用。使用对成熟miRNA特异性的定量实时聚合酶链反应测定，在一组30例具有正常核型的原发性成人急性髓性白血病（AML）中测量选定的一组造血组织特异性miRNA的表达水平。每种miRNA的表达水平与同一白血病的全基因组mRNA表达谱相关。这表明miR-181 a与AML形态学亚型以及先前通过序列分析鉴定为潜在相互作用靶点的基因表达密切相关。其他三种miRNAs，miR-10a，miR-10 b和miR-196 a-1，显示出与HOX基因表达明显相关。
MicroRNAs ( miRNAs) are short single-stranded RNAs that have a potentially important role in gene regulation. Using a quantitative real-time polymerase chain reaction assay specific to the mature miRNA, the expression level of a selected group of haematopoietic tissue-specific miRNAs was measured across a set of 30 primary adult acute myeloid leukaemia (AML) with a normal karyotype. The expression levels of each miRNA were correlated with the genome-wide mRNA expression profiles in the same leukaemias. This revealed that miR-181a correlated strongly with the AML morphological sub-type and with the expression of genes previously identified through sequence analysis as potential interaction targets. Three other miRNAs, miR-10a, miR-10b and miR-196a-1, showed a clear correlation with HOX gene expression.