Clinical feature of intrahepatic B-lymphocytes in chronic hepatitis B.
Clinical feature of intrahepatic B-lymphocytes in chronic hepatitis B.
复制标题
DOI：
10.1155/2014/896864
复制
发表时间：
2014
影响因子：
2
通讯作者：
Poustchi H
中科院分区：
文献类型：
--
作者：
Mohamadkhani A;Naderi E;Sotoudeh M;Katoonizadeh A;Montazeri G;Poustchi H
体液免疫是抵御病毒感染的主要防御机制。然而，慢性乙型肝炎病毒（HBV）携带者肝损伤与B细胞群的关系尚未得到很好的研究。在这项研究中，我们研究了57例乙型肝炎表面抗原（HBsAg）阳性和HBeAg阴性的患者，用免疫组织化学方法测定了CD20（一种表达在B细胞上的细胞表面标记物）在肝活检切片中的表达。肝活检时患者的临床资料来源于患者的医疗记录。log HBV DNA与ALT （r = 0.36, P = 0.006）和HAI（组织学活性指数）总分（r = 0.3, P = 0.02）均有显著相关性。CD20在57例亚膜和膜样肝活检标本中均有表达，其表达与HAI总分（r = 0.32, P = 0.01）和纤维化分期（r = 0.31, P = 0.02）有显著相关性。乙型肝炎病毒易感的B淋巴细胞可能与hbv诱导的肝损伤的免疫介导炎症的发展有关。目前的数据也支持肝脏是潜在的次要淋巴器官之一。
Humoral immunity constitutes major defense mechanism against viral infections. However, the association of hepatic injury and B-cells population in chronic hepatitis B virus (HBV) carriers has not been studied well. In this study, fifty seven hepatitis B surface antigen (HBsAg) positive and HBeAg negative patients were studied to determine the expression of CD20, a cell surface marker expressed on B-cells, in liver biopsy sections using immunohistochemistry. The patients' clinical data at the time of liver biopsy were acquired from their medical records. There was a significant association between log HBV DNA and both ALT (r = 0.36, P = 0.006) and histologic activity index (HAI) total score (r = 0.3, P = 0.02), respectively. The CD20 was expressed in all 57 liver biopsy samples with a submembranous and membranous staining pattern and its expression was significantly associated with HAI total score (r = 0.32, P = 0.01) and stage of fibrosis (r = 0.31, P = 0.02). The susceptible B lymphocytes to hepatitis B virus might be implicated in the development of immune mediated inflammation of HBV-induced hepatic injury. The present data also support that the liver is potentially one of the secondary lymphoid organs.