Resonance Raman Spectroscopy and the Preterm Infant Carotenoid Status
Resonance Raman Spectroscopy and the Preterm Infant Carotenoid Status
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DOI：
10.1097/mpg.0b013e318282a8fe
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发表时间：
2013-05-01
影响因子：
2.9
通讯作者：
Rau, Carrie
中科院分区：
文献类型：
--
作者：
Chan, Gary M.;Chan, Melissa M.;Rau, Carrie
目的：本研究的目的是通过与高效液相色谱法（HPLC）的比较，验证婴儿的无创共振拉曼光谱（RRS）法，并评估母乳或配方奶粉喂养的早产儿的类胡萝卜素状况。方法：在研究的第一阶段，在男性足月婴儿的皮肤上进行共振拉曼测量，并与包皮环切术时采集的组织相关联。每个婴儿的包皮称重，酶消化，提取总类胡萝卜素，并通过HPLC定量。接下来，为了评估早产儿（BW < 1500 g）的类胡萝卜素状态，从喂养开始和每2周直到出院，研究了用人乳或早产儿配方食品喂养的婴儿的皮肤和血清类胡萝卜素。结果：RRS法测定的包皮类胡萝卜素水平与HPLC法测定的血清总类胡萝卜素水平相关（R = 0.52，P <0.01，n = 16）。研究了40名早产儿的类胡萝卜素状况。32名婴儿被喂食母乳，而8名婴儿被喂食未富含类胡萝卜素的早产儿配方奶粉。两个喂养组的妊娠期和出生体重相似。母乳喂养的婴儿比配方奶粉喂养的婴儿有更高的血清总类胡萝卜素浓度和皮肤拉曼计数。皮肤拉曼计数与血清总类胡萝卜素呈正相关（R = 0.44，P = 0.01）。母乳喂养的婴儿的血清总类胡萝卜素浓度和拉曼值在研究期间没有变化，但是，配方奶粉喂养组的血清和皮肤的总类胡萝卜素显着下降在study.Conclusions：RRS婴儿的皮肤可靠地评估总类胡萝卜素状态非侵入性。母乳喂养的早产儿的血清和皮肤类胡萝卜素含量高于配方奶粉喂养的婴儿，这表明配方奶粉喂养的婴儿可能受益于类胡萝卜素的补充。
Objective: The aim of the study was to validate the noninvasive resonance Raman spectroscopy (RRS) method in infants in comparison with the high-performance liquid chromatography (HPLC) method, and to evaluate the carotenoid status in preterm infants fed with mother's milk or formula.Methods: In the first phase of the study, resonance Raman measurements were made on male term infants' skin and correlated with tissue harvested at the time of circumcision. Each baby's foreskin was weighed, enzymatically digested, and the total carotenoids were extracted and quantitated by the HPLC. Next, to evaluate the carotenoid status of preterm infants (BW < 1500 g), the skin and serum carotenoids in infants fed with either human milk or preterm formula were studied from the start of feedings and every 2 weeks until hospital discharge. Skin carotenoids were measured by RRS and the serum total carotenoids by HPLC.Results: Foreskin carotenoid levels measured by RRS correlated with HPLC measurements of total serum carotenoids (R = 0.52, P < 0.01, n = 16). Forty preterm infants were studied for their carotenoid status. Thirty-two infants were fed mother's milk, whereas 8 were fed a preterm infant formula that was not enriched with carotenoids. The gestation and birth weight of the 2 feeding groups were similar. The infants fed human milk had a higher serum total carotenoid concentration and skin Raman counts than formula-fed infants. The skin Raman counts and total serum carotenoid correlated (R = 0.44, P = 0.01). The human milk-fed infants' serum total carotenoid concentrations and Raman values did not change during the study period; however, the formula-fed group's total serum and skin carotenoid decreased significantly during the study.Conclusions: RRS of infant's skin reliably assesses total carotenoid status noninvasively. Human milk-fed preterm infants have higher serum and skin carotenoids than formula-fed infants suggesting that formula-fed infants may benefit from carotenoid supplementation.