HISTONE-H1-DEPENDENT CHROMATIN SUPERSTRUCTURES AND THE SUPPRESSION OF GENE ACTIVITY
HISTONE-H1-DEPENDENT CHROMATIN SUPERSTRUCTURES AND THE SUPPRESSION OF GENE ACTIVITY
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DOI：
10.1016/0092-8674(84)90522-1
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
WEINTRAUB, H
鉴定了含有DNA的染色质颗粒，其大小为20-40千碱基，作为琼脂糖凝胶上的离散实体迁移。随着核酸酶酶切的增加，颗粒在核小体之间的连接区域被切割，但保持完整，可能是由外部组蛋白H1和H5结合在一起。通过杂交分析，在这些颗粒中发现了无活性基因。在含有H1和H5的颗粒中也发现了活性基因（及其侧翼序列），但与无活性的超核小体颗粒相反，活性多核小体颗粒在连接体DNA切割后不会结合在一起。这表明H1在非活性区域与相邻核小体交联，而H1在表达区域的结合方式不同。该结果提出了一种可能性，即被抑制的组织特异性基因的显著抑制程度可能部分地由它们组装到这些无活性的超核小体结构中来决定。
A chromatin particle containing DNA as large as 20-40 kilobases that migrates as a discrete entity on agarose gels was identified. With increasing nuclease digestion, the particle becomes cleaved in the linker regions between nucleosomes, but remains intact, probably held together by the outer histones, H1 and H5. By hybridization analysis, inactive genes are found in these particles. Active genes (and their flanking sequences) are also found in particles containing H1 and H5, but in contrast to inactive supranucleosome particles, active polynucleosome particles are not held together after cleavage of linker DNA. This suggests that H1 cross-links adjacent nucleosomes in inactive regions and that H1 is bound differently in expressed regions. The results raise the possibility that the marked degrees of suppression of repressed, tissue-specific genes may be determined, in part, by their assembly into these inactive supranucleosome structures.