Quantitative phylogenetic assessment of microbial communities in diverse environments
Quantitative phylogenetic assessment of microbial communities in diverse environments
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DOI：
10.1126/science.1133420
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发表时间：
2007-02-23
期刊：
影响因子：
56.9
通讯作者：
Bork, P.
中科院分区：
文献类型：
--
作者：
von Mering, C.;Hugenholtz, P.;Bork, P.
环境群落的分类组成是其生态和功能的重要指标。我们使用了一组蛋白质编码标记基因，从大规模的环境鸟枪测序数据中提取，提供了一个更直接，定量和准确的图片社区组成比传统的核糖体RNA为基础的方法依赖于聚合酶链反应。将来自四个不同环境数据集的标记基因映射到一个参考物种上，结果表明某些群落的进化速度比其他群落快。该方法还能够确定整个微生物分支的首选栖息地，并提供证据表明，这种栖息地偏好往往是显着稳定的时间。
The taxonomic composition of environmental communities is an important indicator of their ecology and function. We used a set of protein-coding marker genes, extracted from large-scale environmental shotgun sequencing data, to provide a more direct, quantitative, and accurate picture of community composition than that provided by traditional ribosomal RNA-based approaches depending on the polymerase chain reaction. Mapping marker genes from four diverse environmental data sets onto a reference species phylogeny shows that certain communities evolve faster than others. The method also enables determination of preferred habitats for entire microbial clades and provides evidence that such habitat preferences are often remarkably stable over time.