Integrated sample desalting, enrichment, and ionization on an omniphobic glass slide for direct mass spectrometry analysis.
Integrated sample desalting, enrichment, and ionization on an omniphobic glass slide for direct mass spectrometry analysis.
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DOI：
10.1002/rcm.9179
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Jing Wang;S. Valentine;Peng Li
中科院分区：
文献类型：
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作者：
Jing Wang;S. Valentine;Peng Li
直接和快速的质谱（MS）分析是许多应用所需要的，包括环境监测，法医分析，化学和生物防御，以及护理点测试。然而，由于离子抑制，使用质谱分析复杂基质中的目标通常需要样品预处理。为了实现快速的质谱分析，需要简单有效的样品处理和电离解决方案。本文报道了一个简单的样品预处理和电离工作流程，在单个玻片上实现了样品的脱盐、富集和电离。方法通过结晶和再溶解诱导分析物与盐的自发分离实现脱盐。在结晶过程中，通过用全疏涂层修饰玻璃表面来实现有效的样品富集。最后，采用振动锐边喷雾电离（VSSI）直接在载玻片上电离目标分子。因此，在质谱分析之前，所有必要的样品操作都在样品玻片上完成。结果首次利用食用色素在全疏玻片上实现了高效的样品富集。通过比较不同处理程序的样品，证明了在1X磷酸盐缓冲盐水（PBS）溶液中检测大环内酯类抗生素的脱盐和富集步骤的好处。最后，定量的大环内酯类抗生素从PBS和血清样品证明。血清样品在2 nM ~ 10 μM的线性范围内，检出限为1 nM。结论建立了一种简单、灵活、低成本、高度集成的MS检测复杂基质中目标分子的工作流程。这种方法将是有价值的许多应用，需要快速和有效的质谱分析复杂的样品。
RATIONALE Direct and rapid mass spectrometry (MS) analysis is desired for many applications including environmental monitoring, forensic analysis, chemical and biological defense, and point-of-care testing. However, sample pretreatment is often necessary for analyzing targets from complex matrices using MS due to ion suppression. To achieve rapid MS analysis calls for simple and efficient solutions for sample processing and ionization. Here, a simple sample pretreatment and ionization workflow is reported, which achieved sample desalting, enrichment, and ionization on a single glass slide. METHODS Desalting is achieved based on crystallization and re-dissolution induced spontaneous separation of analytes and salt. Efficient sample enrichment is achieved during the crystallization process by modifying the glass surface with an omniphobic coating. Finally, vibrating sharp-edge spray ionization (VSSI) is employed to ionize the target molecules directly on the glass slide. Thus, all the necessary sample operations prior to MS analysis are completed on the sample glass slide. RESULTS Efficient sample enrichment on the omniphobic glass slide is first visualized using food dyes. The benefits of the desalting and enrichment steps for detecting macrolide antibiotics in 1X phosphate buffered saline (PBS) solutions are demonstrated by comparing samples with different treatment procedures. Finally, quantification of macrolide antibiotics from PBS and serum samples is demonstrated. A linear range between 2 nM to 10 μM has been achieved for the serum sample with a limit of detection (LOD) of 1 nM. CONCLUSION A simple, flexible, low cost, and highly integrated workflow for detecting target molecules from complex matrices using MS is demonstrated. This method will be valuable to many applications that require rapid and efficient MS analysis of complex samples.