Nitrogen Fixing and Phosphate Mineralizing Bacterial Communities in Sweet Potato Rhizosphere Show a Genotype-Dependent Distribution
Nitrogen Fixing and Phosphate Mineralizing Bacterial Communities in Sweet Potato Rhizosphere Show a Genotype-Dependent Distribution
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DOI：
10.3390/d11120231
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发表时间：
2019-12-01
期刊：
影响因子：
2.4
通讯作者：
Seldin, Lucy
中科院分区：
文献类型：
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作者：
Marques, Joana Montezano;Mateus, Jackeline Rossetti;Seldin, Lucy
我们推测，甘薯的基因型可以影响与根际磷矿化和固氮相关的细菌群落。在种植3个月和6个月后，对IPB-149、IPB-052和IPB-137三个品种的大田甘薯块根进行取样。从根际提取总DNA，分别以碱性磷酸酶编码基因(alp基因)和固氮酶编码基因(nifH基因)为基础，用聚合酶链式反应-变性梯度凝胶电泳法(PCR-DGGE)和实时定量聚合酶链式反应(QPCR)进行分析。基于DGGE的聚类分析表明，植物年龄对IPB-137根际磷矿化相关细菌群落影响不大，但对固氮相关细菌群落影响不大。DGGE指纹图谱的统计分析(排列检验，p
We hypothesize that sweet potato genotypes can influence the bacterial communities related to phosphate mineralization and nitrogen fixation in the rhizosphere. Tuberous roots of field-grown sweet potato from genotypes IPB-149, IPB-052, and IPB-137 were sampled three and six months after planting. The total community DNA was extracted from the rhizosphere and analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and quantitative real-time PCR (qPCR), based on the alkaline phosphatase coding gene (alp gene) and on the nitrogenase coding gene (nifH gene). The cluster analysis based on DGGE showed that plant age slightly influenced the bacterial community related to phosphate mineralization in the rhizosphere of IPB-137, although it did not affect the bacterial community related to nitrogen fixation. The statistical analysis of DGGE fingerprints (Permutation test, p