DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis.
DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis.
复制标题
DOI：
10.1073/pnas.0405568101
复制
发表时间：
2004-09
影响因子：
11.1
通讯作者：
Hui Wang;J. Dunning;A. Huang;Jacqueline A. Nyamwanda;D. Branton
中科院分区：
文献类型：
--
作者：
Hui Wang;J. Dunning;A. Huang;Jacqueline A. Nyamwanda;D. Branton
DNA 和 RNA 样品的广谱分析在不断发展的生物技术领域变得越来越重要。我们表明，纳米孔测量可用于评估核酸制剂的纯度、磷酸化状态和化学完整性。与凝胶电泳和质谱分析相比，DNA 大小和浓度的前所未有的动态范围可以在几分钟内的单个数据采集过程中进行评估。由于分子信息以单分子分辨率进行量化和数字记录，因此可以实时调整系统的灵敏度以检测特定 DNA 物种的痕量。
Broad-spectrum analysis of DNA and RNA samples is of increasing importance in the growing field of biotechnology. We show that nanopore measurements may be used to assess the purity, phosphorylation state, and chemical integrity of nucleic acid preparations. In contrast with gel electrophoresis and mass spectrometry, an unprecedented dynamic range of DNA sizes and concentrations can be evaluated in a single data acquisition process that spans minutes. Because the molecule information is quantized and digitally recorded with single-molecule resolution, the sensitivity of the system can be adjusted in real time to detect trace amounts of a particular DNA species.