CRYSTAL-STRUCTURE OF A 5-FINGER GLI-DNA COMPLEX - NEW PERSPECTIVES ON ZINC FINGERS
CRYSTAL-STRUCTURE OF A 5-FINGER GLI-DNA COMPLEX - NEW PERSPECTIVES ON ZINC FINGERS
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DOI：
10.1126/science.8378770
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发表时间：
1993-09-24
期刊：
影响因子：
56.9
通讯作者：
PABO, CO
中科院分区：
文献类型：
--
作者：
PAVLETICH, NP;PABO, CO
锌指蛋白是首先在转录因子 IIIA (TFIIIA) 中发现的类型，是最大且最重要的 DNA 结合蛋白家族之一。含有来自人类 GLI 癌基因的五个 Zn 指和高亲和力 DNA 结合位点的复合物的晶体结构已在 2.6 埃分辨率下确定。一号手指不接触 DNA。第二到第五指结合在主沟中并包裹 DNA，但缺乏在 Zif268 复合体中观察到的简单、严格的周期性排列。 GLI 的第四指和第五指在保守的九个碱基对区域中进行广泛的碱基接触，并且 DNA 的这一部分具有介于 B-DNA 和 A-DNA 之间的构象。分析 GLI 复合物并将其与 Zif268 进行比较，为 Zn 指 DNA 识别提供了新的视角。
Zinc finger proteins, of the type first discovered in transcription factor IIIA (TFIIIA), are one of the largest and most important families of DNA-binding proteins. The crystal structure of a complex containing the five Zn fingers from the human GLI oncogene and a high-affinity DNA binding site has been determined at 2.6 angstrom resolution. Finger one does not contact the DNA. Fingers two through five bind in the major groove and wrap around the DNA, but lack the simple, strictly periodic arrangement observed in the Zif268 complex. Fingers four and five of GLI make extensive base contacts in a conserved nine base-pair region, and this section of the DNA has a conformation intermediate between B-DNA and A-DNA. Analyzing the GLI complex and comparing it with Zif268 offers new perspectives on Zn finger-DNA recognition.