The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
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DOI：
10.1021/bi8016872
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发表时间：
2009-03-17
期刊：
影响因子：
2.9
通讯作者：
Hall, Kathleen B.
中科院分区：
文献类型：
--
作者：
Clerte, Caroline;Hall, Kathleen B.
PTB（polypyrimidine tract binding protein，多聚嘧啶束结合蛋白）参与细胞核和细胞质中的细胞调节功能。它与内部核糖体进入位点结合，以促进其在帽非依赖性翻译中的使用。它与前mRNA内含子中的多聚嘧啶束结合以抑制外显子的包含。它与mRNA的3′非翻译区结合以稳定信息。这些RNA具有不同的结构，但PTB与所有这些结构结合。在这里，具有结构化或非结构化聚嘧啶段的RNA结合到全长PTB 1蛋白和两个蛋白质亚结构域，每个亚结构域含有两个RNA识别基序。将c-src和GABAA γ2前体mRNA以及丙型肝炎病毒（HCV）3′末端的发夹环与GABAA γ2前体mRNA的单链多聚嘧啶序列进行比较。我们得出结论，PTB 1 RNA结合功能是模块化的：N-末端RRMs优先结合短（U/C）的环中显示的tracts，而RRM 3/RRM 4优先结合较长的灵活的RNA序列。由于它可以结合到短和长的多聚嘧啶束，结构化的或单链的，PTB承担了一个多功能的衔接蛋白的作用，促进RNA的形成：蛋白质调控复合物。
PTB (polypyrimidine tract binding protein) participates in cellular regulatory functions in the nucleus and the cytoplasm. It binds to internal ribosome entry sites to facilitate their use in cap-independent translation. It binds to polypyrimidine tracts in pre-mRNA introns to repress inclusion of exons. It binds to the 3′ untranslated regions of mRNAs to stabilize the message. These RNAs have various structures, yet PTB binds to all of them. Here, RNAs with structured or unstructured polypyrimidine tracts are bound to the full length PTB1 protein and two protein sub-domains, each containing two RNA Recognition Motifs. Hairpin loops from c-src and GABAA γ2 pre-mRNAs and from the 3′ terminus of Hepatitis C Virus (HCV) were compared to a single-stranded polypyrimidine tract from GABAA γ2 pre-mRNA. We conclude that PTB1 RNA binding function is modular: the N-terminal RRMs preferentially bind to short (U/C) tracts displayed in loops, while RRM3/RRM4 preferentially binds to longer flexible RNA sequences. Since it can bind to short and long polypyrimidine tracts, structured or single-stranded, PTB takes on the role of a versatile adaptor protein that facilitates formation of RNA:protein regulatory complexes.
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影响因子：
5.3
作者：
Hamilton, BJ;Genin, A;Rigby, WFC
通讯作者：
Rigby, WFC
影响因子：
14.9
作者：
GHETTI, A;PINOLROMA, S;DREYFUSS, G
通讯作者：
DREYFUSS, G
DOI：
10.1073/pnas.90.16.7642
发表时间：
1993-08-15
影响因子：
11.1
作者：
HELLEN, CUT;WITHERELL, GW;WIMMER, E
通讯作者：
WIMMER, E
影响因子：
12.3
作者：
Gama-Carvalho, Margarida;Barbosa-Morais, Nuno L.;Carmo-Fonseca, Maria
通讯作者：
Carmo-Fonseca, Maria
影响因子：
4.5
作者：
Clerte, C;Hall, KB
通讯作者：
Hall, KB