DETECTION OF HERPES-SIMPLEX VIRUS-SPECIFIC DNA-SEQUENCES IN LATENTLY INFECTED MICE AND IN HUMANS
DETECTION OF HERPES-SIMPLEX VIRUS-SPECIFIC DNA-SEQUENCES IN LATENTLY INFECTED MICE AND IN HUMANS
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DOI：
10.1128/jvi.57.2.446-455.1986
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发表时间：
1986-02-01
影响因子：
5.4
通讯作者：
WILDY, P
中科院分区：
文献类型：
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作者：
EFSTATHIOU, S;MINSON, AC;WILDY, P
通过Southern杂交分析，在潜伏感染的小鼠的中枢和外周神经系统组织中都检测到了单纯疱疹病毒特异性DNA序列。我们已经检测到与连接片段相对应的病毒特异性序列，但没有检测到基因组末端，这是Rock和Fraser首先观察到的(自然[伦敦]302：523-525,1983)。这种无穷无尽的单纯疱疹病毒DNA在4个月的时间里在小鼠神经组织中的分析在定性和定量上都是稳定的。此外，对从人类三叉神经节提取的DNA的检测表明，到目前为止，单纯疱疹病毒DNA以类似于在小鼠模型系统中发现的“无穷无尽”的形式存在。对潜伏感染的小鼠脑干和人类三叉神经DNA的进一步限制性内切酶分析表明，这种无穷无尽的单纯疱疹病毒DNA存在于所有四种异构体中。
Herpes simplex virus-specific DNA sequences have been detected by Southern hybridization analysis in both central and peripheral nervous system tissues of latently infected mice. We have detected virus-specific sequences corresponding to the junction fragment but not the genomic termini, an observation first made by Rock and Fraser (Nature [London] 302:523-525, 1983). This "endless" herpes simplex virus DNA is both qualitatively and quantitatively stable in mouse neural tissue analyzed over a 4-month period. In addition, examination of DNA extracted from human trigeminal ganglia has shown herpes simplex virus DNA to the present in an "endless" form similar to that found in the mouse model system. Further restriction enzyme analysis of latently infected mouse brainstem and human trigeminal DNA has shown that this "endless" herpes simplex virus DNA is present in all four isomeric configurations.