INDUCTION OF NUCLEAR TRANSPORT WITH A SYNTHETIC PEPTIDE HOMOLOGOUS TO THE SV40 T-ANTIGEN TRANSPORT SIGNAL
INDUCTION OF NUCLEAR TRANSPORT WITH A SYNTHETIC PEPTIDE HOMOLOGOUS TO THE SV40 T-ANTIGEN TRANSPORT SIGNAL
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DOI：
10.1016/0092-8674(86)90883-4
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发表时间：
1986-08-15
期刊：
影响因子：
64.5
通讯作者：
KENNEDY, RC
中科院分区：
文献类型：
--
作者：
LANFORD, RE;KANDA, P;KENNEDY, RC
开发了一种用于分析蛋白质转运至细胞核的系统。将与合成肽交联的载体蛋白显微注射到哺乳动物细胞的细胞质中，并通过固定细胞的免疫荧光染色来评估蛋白质转运。含有与 SV40 T 抗原同源的 7 个氨基酸的 13 聚体合成肽能够诱导核转运，但当蛋白质与与核转运缺陷型 T 抗原同源的合成肽偶联时，没有观察到转运。有效运输的最大的蛋白质-肽缀合物是铁蛋白（465,000 先生）。转运速率受每个载体蛋白分子的肽数量的影响，并且在某种程度上受载体蛋白大小的影响。一些缀合物的运输在室温下 15 分钟内几乎完成。
A system was developed for the analysis of protein transport to the nucleus. Carrier proteins cross-linked to synthetic peptides were microinjected into the cytoplasm of mammalian cells, and protein transport was evaluated by immunofluorescence staining of fixed cells. A 13-mer synthetic peptide containing seven amino acids homologous to SV40 T antigen was capable of inducing nuclear transport, but no transport was observed when proteins were coupled with a synthetic peptide homologous to a nuclear-transport-defective T antigen. The largest protein-peptide conjugate efficiently transported was ferritin (Mr 465,000). The rate of transport was influenced by the number of peptides per molecule of carrier protein and, to some degree, by the size of the carrier protein. Transport of some conjugates was almost complete in 15 min at room temperature.