A role of melanin-concentrating hormone producing neurons in the central regulation of paradoxical sleep.
A role of melanin-concentrating hormone producing neurons in the central regulation of paradoxical sleep.
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DOI：
10.1186/1471-2202-4-19
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发表时间：
2003-09-09
期刊：
影响因子：
2.4
通讯作者：
Luppi, PH
中科院分区：
文献类型：
--
作者：
Verret, L;Goutagny, R;Fort, P;Cagnon, L;Salvert, D;Léger, L;Boissard, R;Salin, P;Peyron, C;Luppi, PH
含有黑色素浓缩激素（MCH）和下丘脑分泌素（或食欲素）的肽能神经元混杂在中间带、皮层周围核和下丘脑外侧区。这两种类型的神经元都与能量稳态和体重的综合调节有关。下丘脑分泌素神经元也参与睡眠-觉醒调节和嗜睡症。因此，我们试图确定下丘脑分泌素和MCH神经元是否在睡眠剥夺后反弹期间表达Fos与增强的矛盾睡眠（PS或REM睡眠）有关。接下来，我们比较了脑室注射MCH和NaCl对睡眠阶段数量的影响，以进一步确定MCH神经元是否在PS调节中发挥积极作用。通过对三组大鼠（PS控制组、PS剥夺组和PS恢复组）的下丘脑泌素、MCH和Fos联合免疫染色，我们发现在PS期间，MCH而非下丘脑泌素神经元强烈活跃。此外，我们表明，ICV给药MCH诱导PS（高达200%）和慢波睡眠（高达70%）数量的剂量依赖性增加。这些结果表明MCH是一个强大的催眠因素。MCH神经元可能通过其在中枢神经系统的广泛投射而在PS状态中发挥关键作用。
Peptidergic neurons containing the melanin-concentrating hormone (MCH) and the hypocretins (or orexins) are intermingled in the zona incerta, perifornical nucleus and lateral hypothalamic area. Both types of neurons have been implicated in the integrated regulation of energy homeostasis and body weight. Hypocretin neurons have also been involved in sleep-wake regulation and narcolepsy. We therefore sought to determine whether hypocretin and MCH neurons express Fos in association with enhanced paradoxical sleep (PS or REM sleep) during the rebound following PS deprivation. Next, we compared the effect of MCH and NaCl intracerebroventricular (ICV) administrations on sleep stage quantities to further determine whether MCH neurons play an active role in PS regulation. Here we show that the MCH but not the hypocretin neurons are strongly active during PS, evidenced through combined hypocretin, MCH, and Fos immunostainings in three groups of rats (PS Control, PS Deprived and PS Recovery rats). Further, we show that ICV administration of MCH induces a dose-dependant increase in PS (up to 200%) and slow wave sleep (up to 70%) quantities. These results indicate that MCH is a powerful hypnogenic factor. MCH neurons might play a key role in the state of PS via their widespread projections in the central nervous system.
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影响因子：
64.5
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通讯作者：
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