SMAD expression in the testis: An insight into BMP regulation of spermatogenesis
SMAD expression in the testis: An insight into BMP regulation of spermatogenesis
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DOI：
10.1002/dvdy.21401
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Loveland, Kate Lakoski
中科院分区：
文献类型：
--
作者：
Itman, Catherine;Loveland, Kate Lakoski
骨形态发生蛋白（BMPs）是转化生长因子- β超家族的成员，广泛影响男性生育能力的建立，影响整个胎儿和出生后的生殖细胞和体细胞。BMP信号由沙拉蛋白传递，转录因子在配体刺激下转运到细胞核。我们发现睾丸中的BMP信号可能受BMP反应性和抑制性smad的选择性表达调控，其表达在第一波和成年精子发生期间有所不同。Smad1、Smad5、Smad8、Smad4、Smad6和Smad7的表达在睾丸发育过程中普遍存在，但在成年后具有细胞特异性。此外，SMAD6蛋白在精子发生时的表达调控表明，精原亚群对配体的反应存在差异。体外，未成熟的Sertoli细胞和精原细胞通过SMAD1、SMAD5和SMAD8转导BMP2和BMP4信号。基于这些发现，我们推断这些数据来解释BMP突变睾丸表型的沙拉可用性的信号转导。
Bone morphogenetic proteins (BMPs), members of the transforming growth factor-beta superfamily, extensively influence events that establish male fertility, affecting germ cells and somatic cells throughout fetal and postnatal life. BMP signals are relayed by SALAD proteins, transcription factors that translocate to the nucleus upon ligand stimulation. We show that BMP signaling in the testis may be regulated by selective expression of BMP-responsive and inhibitory SMADs, with expression differing between the first wave and adult spermatogenesis. Smad1, Smad5, Smad8, Smad4, Smad6, and Smad7 expression is ubiquitous during testis development but becomes cell-specific in the adult. Furthermore, regulated SMAD6 protein expression at the onset of spermatogenesis suggests differential responsiveness of spermatogonial subpopulations to ligands. In vitro, immature Sertoli cells and spermatogonia transduce BMP2 and BMP4 signals by means of SMAD1, SMAD5, and SMAD8. Based on these findings, we extrapolate these data to interpret BMP mutant testis phenotypes in terms of SALAD availability for signal transduction.