Hyperthermia Induced by Magnetic Nanoparticles Improves the Effectiveness of the Anticancer Drug cis-Diamminedichloroplatinum
Hyperthermia Induced by Magnetic Nanoparticles Improves the Effectiveness of the Anticancer Drug cis-Diamminedichloroplatinum
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DOI：
10.1166/jnn.2011.3821
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发表时间：
2011-05-01
影响因子：
--
通讯作者：
Torres-Lugo, Madeline
中科院分区：
文献类型：
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作者：
Lee, Jason S.;Rodriguez-Luccioni, Hector L.;Torres-Lugo, Madeline
在人结肠腺癌细胞 (Caco-2) 中研究了磁流体热疗 (MFH) 增强顺铂的细胞毒性。采用基于羧甲基葡聚糖功能化的氧化铁的纳米颗粒平台来产生纳米级的热量。为了评估热疗和抗癌药物顺式二氨二氯铂（通常称为顺铂 (CIS)）的协同效应，在三种不同的组合热疗和 CIS 暴露序列后 24、48 和 72 小时测量细胞活力。这些包括热疗或磁流体热疗之前的 CIS 孵育、仅在热疗或 MFH 期间的 CIS 暴露以及热疗或 MFH 后的额外 CIS 孵育。对于两种热疗治疗，在热疗治疗后额外的 CIS 孵育似乎比之前的 CIS 孵育更有效。活力数据还表明，在相同温度下，MFH 联合 CIS 明显比热水热疗更有效。发现比计算的 IC50 低一个数量级的 CIS 浓度对于降低细胞活力非常有效。如此巨大的差异表明 MFH 可能增强 CIS 的被动转运。
The cytotoxic enhancement of cisplatin by magnetic fluid hyperthermia (MFH) was investigated in human colon adenocarcinoma cells (Caco-2). A nanoparticle platform based on iron oxide functionalized with carboxymethyl dextran was employed to produce heat at the nanoscale. To assess the synergistic effect of hyperthermia and the anticancer drug cis-Diamminedichloroplatinum, commonly known as cisplatin (CIS), cell viability was measured 24, 48, and 72 hours after three different combined hyperthermia and CIS exposure sequences. These included CIS incubation prior to hyperthermia or magnetic fluid hyperthermia, CIS exposure only during hyperthermia or MFH, and additional CIS incubation following hyperthermia or MFH. Additional incubation of CIS after hyperthermia treatment appears to be more effective than prior CIS incubation for both hyperthermia treatments. Viability data also indicated that MFH combined with CIS is significantly more effective than hot water hyperthermia at the same temperature. A CIS concentration an order of magnitude lower than the calculated IC50 was found to be very effective in reducing cell viability. Such dramatic differences suggest that MFH may enhance the passive transport of CIS.