Spatiotemporal expression of MYD88 gene in pigs from birth to adulthood.
Spatiotemporal expression of MYD88 gene in pigs from birth to adulthood.
复制标题
猪出生至成年期间MYD88基因的时空表达
DOI：
10.1590/1678-4685-gmb-2017-0014
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发表时间：
2018-01
影响因子：
2.1
通讯作者：
Bao W
中科院分区：
文献类型：
--
作者：
Gan L;Qin W;Wu S;Wu S;Bao W
摘要MYD88在机体对感染的免疫应答中起重要作用。为了分析MYD88基因在猪不同发育阶段的表达情况，我们采用了实时荧光定量聚合酶链式反应技术。MyD88在所有受检组织中均有表达。MyD88在脾、肺和胸腺中的表达在7日龄至14日龄达到最高值，此后逐渐下降。在28日龄之前，淋巴结中有较高的表达，断奶后呈下降趋势，成年猪中表达水平稳定较低。MyD88在35日龄以前在小肠(十二指肠、空肠和回肠)中有较高的表达，在7~14日龄达到最高值。MyD88在断奶后的小肠中的表达下降，并在成年期保持相对较低的水平。本研究结果提示，断奶应激和免疫系统的发育可能与MYD88的表达调控呈正相关。此外，本研究还为MYD88的高表达可能减轻梅山猪的断奶应激，提高其抗病能力提供了证据。
Abstract MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The results of this study suggest that weaning stress and development of the immune system might be positively correlated with MYD88 expression regulation. Moreover, this study provided evidence that the high expression of MYD88 may diminish weaning stress and increase disease resistance in Meishan pigs.