Interaction of nucleolin with an evolutionarily conserved pre-ribosomal RNA sequence is required for the assembly of the primary processing complex
Interaction of nucleolin with an evolutionarily conserved pre-ribosomal RNA sequence is required for the assembly of the primary processing complex
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DOI：
10.1074/jbc.m002350200
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发表时间：
2000-06-23
影响因子：
4.8
通讯作者：
Bouvet, P
中科院分区：
文献类型：
--
作者：
Ginisty, H;Serin, G;Bouvet, P
前体核糖体RNA(Pre-rRNA)的第一次加工事件发生在5‘外部转录间隔区内。这种初级加工需要切割位点下游保守的CIA作用RNA序列和几个核酸(小核仁RNAs)和蛋白质反式作用因子，包括核仁蛋白，这是一种主要的核仁蛋白，需要核仁特异性地与前rRNA相互作用才能在体外进行加工。非洲爪哇和仓鼠核仁素与相同的前rRNA位点相互作用，并刺激小鼠细胞提取物的加工活动。核苷酸与加工的相互作用需要一个高度保守的11个核苷酸序列，位于加工部位之后的5-6个核苷酸。在体外核仁选择实验中，已经确定了一个含有UCGA基序的RNA序列，该序列存在于11个核苷酸的保守序列中，核仁素与前rRNA的相互作用是形成活性加工复合体所必需的。我们的研究结果表明，核仁素是核糖体前核糖核粒组装和成熟的关键因素。
The first processing event of the precursor ribosomal RNA (pre-rRNA) takes place within the 5' external transcribed spacer. This primary processing requires conserved cia-acting RNA sequence downstream from the cleavage site and several nucleic acids (small nucleolar RNAs) and proteins trans-acting factors including nucleolin, a major nucleolar protein, The specific interaction of nucleolin with the pre-rRNA is required for processing in vitro. Xenopus laevis and hamster nucleolin interact with the same pre-rRNA site and stimulate the processing activity of a mouse cell extract. A highly conserved 11-nucleotide sequence located 5-6 nucleotides after the processing site is required for the interaction of nucleolin and processing. In vitro selection experiments with nucleolin have identified an RNA sequence that contains the UCGA motif present in the 11-nucleotide conserved sequence, The interaction of nucleolin with pre-rRNA is required for the formation of an active processing complex. Our findings demonstrate that nucleolin is a key factor for the assembly and maturation of pre-ribosomal ribonucleoparticles.