Characterization and in vivo distribution of influenza-virus-specific T-lymphocytes in the murine respiratory tract.
Characterization and in vivo distribution of influenza-virus-specific T-lymphocytes in the murine respiratory tract.
复制标题
小鼠呼吸道中流感病毒特异性 T 淋巴细胞的表征和体内分布。
DOI：
10.1164/arrd.1987.135.1.245
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Bienenstock,J
中科院分区：
文献类型：
--
作者：
McDermott,MR;Lukacher,AE;Braciale,VL;Braciale,TJ;Bienenstock,J
有报道称，在长期连续培养中保持的抗原特异的小鼠T淋巴细胞克隆群体在过继转移后显示出功能活性。目前关于这些同质的、功能细胞群体的活体分布的数据很少。这些信息对于理解T淋巴细胞发挥效应器活性的机制很重要。我们检测了2个克隆的流感病毒特异性T淋巴细胞。一个克隆是Lyt-2+，L3T4−，I类MHC限制性的细胞溶解T淋巴细胞亚群。另一个克隆是辅助/放大T细胞亚群的Lyt-2−，L3T4+，II类MHC限制性克隆。这两个克隆在过继转移后都能促进致命的肺部流感感染的康复。使用[~3H]胸腺嘧啶核苷标记的细胞群，我们检查了这些细胞在不同器官的组织切片中的分布。这些克隆的细胞群体优先保留在未感染和感染流感病毒的动物的肺中。这种保留与细胞的病毒抗原特异性无关，但可能取决于克隆的表型。一旦这些细胞滞留在肺内，它们就会穿过支气管固有层，进入上皮和肺腔。讨论了这些观察对活体淋巴细胞功能的意义。
Cloned populations of antigen-specific, murine T-lymphocytes, maintained in long-term continuous culture, have been reported to exhibit functional activity upon adoptive transfer. Few data are currently available on thein vivodistribution of these homogenous, functional cell populations. Such information is important to understanding the mechanisms by which T-lymphocytes exert their effector activity. We examined 2 cloned populations of influenza virus-specific T-lymphocytes. One clone was a Lyt-2+, L3T4−, class I MHC-restricted, cytolytic T-lymphocyte subset. The other clone was a Lyt-2−, L3T4+, Class II MHC-restricted clone of the helper/amplifier T-cell subset. Both clones promote recovery from lethal pulmonary influenza infection upon adoptive transfer. Using cell populations labeled with [3H]thymidine, we examined the distribution of these cells in tissue sections from various organs. These cloned cell populations were preferentially retained in the lungs of uninfected and influenza-virus-infected animals. This retention is independent of the cell's viral antigenic specificity, but may be dependent on the phenotype of the clone. Once retained in the lungs, these cells migrated across the bronchial lamina propria and entered the epithelium and pulmonary lumen. The significance of these observations forin vivoT-lymphocyte functions is discussed.