Pressure increases oxygen affinity of whole blood and erythrocyte suspensions.
Pressure increases oxygen affinity of whole blood and erythrocyte suspensions.
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压力增加全血和红细胞悬浮液的氧亲和力。
DOI：
10.1152/jappl.1986.61.2.486
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Morin,RA
中科院分区：
文献类型：
--
作者：
Reeves,RB;Morin,RA
本文用新发展的技术研究了静水压（HP）对全血（WB）和红细胞悬液血红蛋白（Hb）O2亲和力的影响。在5 HP（1、26、51、76和126 ATA）下，在37 ℃下，在人WB或红细胞的薄膜上进行血红蛋白被O2半饱和时的O2分压（P50）测量。WB的CO2分压为28或57托（膜pH 7.51或7.31）。HP可增加红细胞与WB的亲和力。在pH7.0的三羟甲基氨基甲烷缓冲液中，红细胞P50（1 ATA）/P50（51 ATA）的比值（r）为1.089（P <0.01）。WB P50随HP以-3.3 X 10（-2）Torr X atm-1的速率降低;较高HP与1 ATA时P50的变化非常显著（P <0.01）。HP对CO2玻尔系数没有影响。惰性气体的选择，N2与氦气（He），没有影响。在溶血WB中，用76 ATA的HP测量P50的降低，得到的r为1.15，与WB中发现的一样大或更大，表明不涉及唐南平衡改变。在WB中未发现HP对具有正常（5 mmol/l红细胞）2，3-二磷酸甘油酸（DPG）的红细胞的P50与具有低于5%正常DPG的红细胞的P50的比率的影响;即，压力对DPG对P50的独立影响没有影响。WB测量的Hb O2吸收在模拟生理条件下的特点是净减少部分摩尔体积氧合30-35 ml/mol Hb 4。
Effect of hydrostatic pressure (HP) on whole blood (WB) or erythrocyte suspension hemoglobin (Hb) O2 affinity has been studied using newly developed techniques. O2 partial pressure at which hemoglobin is half-saturated with O2 (P50) measurements were made at 5 HP (1, 26, 51, 76, and 126 ATA) on thin films of human WB or erythrocytes at 37 degrees C. CO2 partial pressure of WB was either 28 or 57 Torr (film pH 7.51 or 7.31). HP increased affinity of erythrocytes and WB. For erythrocytes in tris(hydroxymethyl)aminomethane buffer, the ratio (r) of P50 (1 ATA)/P50 (51 ATA) was 1.089 (P less than 0.01) at pH 7.0. WB P50 decreased with HP at a rate of -3.3 X 10(-2) Torr X atm-1; change in P50 at higher HP vs. 1 ATA was highly significant (P less than 0.01). No effect of HP was seen on the CO2 Bohr coefficient. Inert gas choice, N2 vs. helium (He), had no effect. Measurement of decrease of P50 with HP at 76 ATA in hemolyzed WB gave an r of 1.15, as great or greater than that found in WB, indicates that Donnan equilibrium alteration is not involved. No effect of HP was found in WB on the ratio of P50 of erythrocytes with normal (5 mmol/l erythrocytes) 2,3-diphosphoglycerate (DPG) to P50 of erythrocytes with less than 5% of normal DPG; i.e., no effect of pressure was seen on the independent influence of DPG on P50. WB measurements of Hb O2 uptake under simulated physiological conditions are characterized by a net decrease in partial molal volume on oxygenation of 30–35 ml/mol Hb4.